Gyeongnam International Foreign School, Sacheon, Republic of Korea.
Beckman Laser Institute-Korea, Cheonan, Republic of Korea.
J Photochem Photobiol B. 2023 Sep;246:112770. doi: 10.1016/j.jphotobiol.2023.112770. Epub 2023 Aug 9.
In this study, the effect of photobiomodulation (PBM) treatment using 630 nm light emitting diode (LED) array (continuous wave type, 10 mW power) on tonsil-derived mesenchymal stem cells (TMSCs) and its interaction with RAW 264.7 macrophage cells via co-culture in vitro were investigated. PBM treatment was used as a priming method for TMSCs to improve therapeutic efficacy. TMSCs were subjected to multi-dose PBM treatments before co-culture with M1 activated (1 μg/mL lipopolysaccharide, LPS) macrophage cells with total energy doses of 0, 15, 30, and 60 J. Irradiation set at 15 J (1500 s treatment time) was performed once, twice for 30 J, and four times for 60 J in an incubator kept at 37 °C and 5% CO. No significant anti-inflammatory response was observed for TMSCs co-cultured with macrophage cells without PBM. But PBM treatment of TMSCs with 630 nm LED array at 30 J reduced expression of inducible nitric oxide synthase, iNOS (M1) and increased expression of Arginase-1, Arg-1 (M2) phenotype macrophage markers. Anti-inflammatory cytokine interleukin-1 receptor antagonist (IL-1RA) gene expression also increased significantly. Based on the results, PBM priming of TMSCs supports M2 macrophage polarization. PBM can be used to improve the therapeutic efficacy of TMSCs for potential applications in oral mucositis and wound healing.
在这项研究中，我们研究了 630nm 发光二极管（LED）阵列（连续波型，10mW 功率）的光生物调节（PBM）治疗对扁桃体间充质干细胞（TMSCs）的影响，以及其与 RAW 264.7 巨噬细胞在体外共培养时的相互作用。PBM 治疗被用作 TMSC 的初始方法，以提高治疗效果。在与 M1 激活（1μg/mL 脂多糖，LPS）巨噬细胞共培养之前，TMSC 接受多次 PBM 治疗，总能量剂量为 0、15、30 和 60J。在 37°C 和 5%CO2 的孵育箱中，以 15J（1500s 治疗时间）进行一次照射，30J 进行两次照射，60J 进行四次照射。未经 PBM 处理的与巨噬细胞共培养的 TMSC 未观察到明显的抗炎反应。但是，630nm LED 阵列对 TMSC 进行 30J 的 PBM 处理可降低诱导型一氧化氮合酶（iNOS，M1）的表达，并增加精氨酸酶-1（Arg-1，M2）表型巨噬细胞标志物的表达。抗炎细胞因子白细胞介素 1 受体拮抗剂（IL-1RA）基因表达也显著增加。基于这些结果，TMSC 的 PBM 预处理支持 M2 巨噬细胞极化。PBM 可用于提高 TMSC 的治疗效果，有望应用于口腔黏膜炎和伤口愈合。
