Signal Transduction Laboratory, Department of Biochemistry, Quaid-i-Azam University Islamabad, Islamabad, 45320, Pakistan.
Department of Biochemistry, Quaid-I-Azam University Islamabad, Islamabad, 45320, Pakistan.
J Cancer Res Clin Oncol. 2023 Nov;149(16):14565-14575. doi: 10.1007/s00432-023-05247-3. Epub 2023 Aug 14.
Breast cancer is an abnormal division of breast cells. Bisphenol A (BPA), an environmental toxicant, is identified as an emerging risk factor for breast cancer development. However, to the best of our knowledge, no previous study has investigated the BPA levels in breast cancer patients in Pakistan. The present study sought to explore the role of BPA in tumor growth among the Pakistani population.
The levels of BPA were analyzed in the serum samples of breast cancer patients and controls by using HPLC. To elucidate the role of BPA to initiate tumorigenic events in breast tissue different biochemical assays along with expression analysis of tumor markers were performed.
The level of BPA in the serum samples of breast cancer patients was significantly higher than control. Histological analysis of breast cancer tissue samples revealed distinct subtypes of tumor, such as ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC). There was a significant increase in ROS level while a significant decrease in the levels of superoxide dismutase (SOD) and catalase (CAT) enzymes in malignant breast tissue samples as compared to control tissue samples. We found upregulated expression of p53, ZEB1 and WNT1 genes at mRNA level in malignant breast tissue samples by 17 folds, 328 folds and 35 folds, respectively. p53 protein expression in malignant breast tissue samples was also enhanced at the translational level.
Current findings suggest a relationship between BPA and the progression of breast cancer among the Pakistani population.
乳腺癌是乳腺细胞的异常分裂。双酚 A（BPA）作为一种环境毒物，被认为是乳腺癌发展的一个新兴风险因素。然而，据我们所知，以前没有研究调查过巴基斯坦乳腺癌患者的 BPA 水平。本研究旨在探讨 BPA 在巴基斯坦人群中对肿瘤生长的作用。
采用高效液相色谱法（HPLC）分析乳腺癌患者和对照组血清样本中的 BPA 水平。为了阐明 BPA 引发乳腺组织肿瘤发生事件的作用，进行了不同的生化分析以及肿瘤标志物的表达分析。
乳腺癌患者血清样本中的 BPA 水平明显高于对照组。乳腺癌组织样本的组织学分析显示出不同的肿瘤亚型，如导管原位癌（DCIS）和浸润性导管癌（IDC）。与对照组组织样本相比，恶性乳腺组织样本中的 ROS 水平显著升高，而超氧化物歧化酶（SOD）和过氧化氢酶（CAT）酶的水平显著降低。我们发现恶性乳腺组织样本中 p53、ZEB1 和 WNT1 基因的 mRNA 水平分别上调了 17 倍、328 倍和 35 倍，而 p53 蛋白在恶性乳腺组织样本中的翻译水平也得到了增强。
目前的研究结果表明 BPA 与巴基斯坦人群中乳腺癌的进展之间存在关联。
