Blanchetot A, Price M, Jeffreys A J
Eur J Biochem. 1986 Sep 15;159(3):469-74. doi: 10.1111/j.1432-1033.1986.tb09909.x.
Seal myoglobin (Mb) exons 1 and 3 were used as probes to isolate the functional mouse Mb gene. This gene has a very low level of Mb expression in skeletal muscle. Although it is shorter, the mouse Mb gene displays the common organisation found in human and seal Mb genes. In addition, we have defined blocks of conserved sequences in the 5' flanking region by comparison with other mammalian Mb genes. Moreover, about 10(3) bases upstream of the cap site we identified a repetitive B1 element directly associated with two overlapping open reading frames, containing a putative polyadenylation signal. A polypyrimidine-rich region has also been located upstream from the gene.
用海豹肌红蛋白（Mb）外显子1和3作为探针来分离功能性小鼠Mb基因。该基因在骨骼肌中的Mb表达水平非常低。尽管小鼠Mb基因较短，但它具有在人类和海豹Mb基因中常见的结构。此外，通过与其他哺乳动物Mb基因比较，我们在5'侧翼区域定义了保守序列块。而且，在帽位点上游约10³个碱基处，我们鉴定出一个与两个重叠开放阅读框直接相关的重复B1元件，其中包含一个推定的聚腺苷酸化信号。在该基因上游还定位到一个富含多嘧啶的区域。
