Suna He -, Haofeng Pan -, Xingyue Qian -, Junyang Zhang -, Runfang Zhang -
Department of Pharmacy, College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, People's Republic of China.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, People's Republic of China.
Pak J Pharm Sci. 2023 May;36(3):895-900.
Exosomes are focused as natural drug delivery vehicles with the advantages of biocompatible, biodegradable and non-immunogenic. However, the low yield of exosomes is one of the challenges that constrain its application. Mimetic exosomes (m-Exo) are the novel cell-derived nano-carriers with similar properties to exosomes and the substantially greater yield is attractive. Herein, in order to evaluate the feasibility of m-Exo as drug delivery vehicles, M-Exo derived from red blood cells were prepared via ultrasonic method, characterized and loaded with doxorubicin (DOX-m-Exo). The preparation methods of DOX-m-Exo were optimized, drug loading as the evaluated index. The drug release and cytotoxicity in vitro were studied by dialysis method and MTT method, respectively. The results demonstrated that m-Exo successfully prepared showed spherical morphology and the particle size and Zeta potential were 161nm with a narrow PDI 0.238 and -25.7mV, respectively, the mixed solution of 0.085% NaCl and 0.47% glucose as the dilution medium. The drug loading of DOX-m-Exo prepared by electroporation was up to 57µg/ml. What's more, DOX-m-Exo displayed sustained release behavior and similar cytotoxicity against MCF-7 cells to DOX solution. In conclusion, the studies laid a certain foundation for m-Exo serving as novel and promising drug delivery vehicles.
外泌体作为天然药物递送载体受到关注，具有生物相容性、可生物降解和非免疫原性等优点。然而，外泌体产量低是限制其应用的挑战之一。模拟外泌体（m-Exo）是一种新型的细胞衍生纳米载体，具有与外泌体相似的性质，且产量显著更高，颇具吸引力。在此，为了评估m-Exo作为药物递送载体的可行性，通过超声法制备了源自红细胞的m-Exo，对其进行表征并装载阿霉素（DOX-m-Exo）。以载药量为评价指标，对外泌体载药阿霉素（DOX-m-Exo）的制备方法进行优化。分别采用透析法和MTT法研究其体外药物释放和细胞毒性。结果表明，成功制备的m-Exo呈球形，以0.085% NaCl和0.47%葡萄糖混合溶液为稀释介质时，粒径为161nm，PDI窄，为0.238，Zeta电位为-25.7mV。通过电穿孔法制备的DOX-m-Exo载药量高达57μg/ml。此外，DOX-m-Exo表现出缓释行为，对MCF-7细胞的细胞毒性与阿霉素溶液相似。总之，这些研究为m-Exo作为新型且有前景的药物递送载体奠定了一定基础。
