Tremp Annie Z, Saeed Sadia, Dessens Johannes T
Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
Front Ecol Evol. 2023 Jun 28;11. doi: 10.3389/fevo.2023.1216385.
Membrane-located NAD(P) transhydrogenase (NTH) catalyses reversible hydride ion transfer between NAD(H) and NADP(H), simultaneously translocating a proton across the membrane. The enzyme is structurally conserved across prokaryotes and eukaryotes. In heterotrophic bacteria NTH proteins reside in the cytoplasmic membrane, whereas in animals they localise in the mitochondrial inner membrane. Eukaryotic NTH proteins exists in two distinct configurations (isoforms) and have non-mitochondrial functions in unicellular eukaryotes like , the causative agent of malaria. In this study, we carried out a systematic analysis of genes across eukaryotic life to determine its prevalence and distribution of isoforms. The results reveal that NTH is found across all major lineages, but that some organisms, notably plants, lack genes altogether. Isoform distribution and phylogenetic analysis reveals different gene loss scenarios in apicomplexan lineages, which sheds new light on the evolution of the Piroplasmida and .
膜定位的NAD(P)转氢酶（NTH）催化NAD(H)和NADP(H)之间可逆的氢负离子转移，同时使一个质子跨膜转运。该酶在原核生物和真核生物中结构保守。在异养细菌中，NTH蛋白存在于细胞质膜中，而在动物中它们定位于线粒体内膜。真核NTH蛋白以两种不同的构型（异构体）存在，并且在单细胞真核生物如疟疾病原体 中具有非线粒体功能。在本研究中，我们对真核生物中的 基因进行了系统分析，以确定其异构体的普遍性和分布。结果表明，NTH存在于所有主要谱系中，但一些生物，特别是植物，完全缺乏 基因。异构体分布和系统发育分析揭示了顶复门类谱系中不同的 基因丢失情况，这为梨形虫纲和 的进化提供了新的线索。
