College of Textile and Clothing Engineering, Soochow University, Suzhou 215021, People's Republic of China.
Key Laboratory of Textile Industry for Silk Products in Medical and Health Use, Soochow University, Suzhou 215127, People's Republic of China.
Biomed Mater. 2023 Sep 6;18(6). doi: 10.1088/1748-605X/acf0ae.
Compared with single-channel nerve conduits, multichannel artificial nerve conduits are more beneficial for repairing damaged peripheral nerves of long-distance nerve defects. Multichannel nerve conduits can be fabricated by the mold method and the electrospinning method but with disadvantages such as low strength and large differences in batches, while the braiding method can solve this problem. In this study, polylactic acid yarns were used as the braiding yarn, and the number of spindles during braiding was varied to achieve 4, 5, 6, 7 and 8 multichannel artificial nerve conduits. A mathematical model of the number of braiding yarn spindles required to meet certain size specification parameters of the multichannel conduit was established. The cross-sectional morphology and mechanical properties of the conduits were characterized by scanning electron microscopy observation and mechanical testing; the results showed that the multichannel structure was well constructed; the tensile strength of the multichannel conduit was more than 30 times that of the rabbit tibial nerve. The biocompatibility of the conduit was tested; thecell culture results proved that the braided multichannel nerve conduits were nontoxic to Schwann cells, and the cell adhesion and proliferation were optimal in the 4-channel conduit among the multichannel conduits, which was close to the single-channel conduit.
与单通道神经导管相比，多通道人工神经导管更有利于修复长距离神经缺损的受损周围神经。多通道神经导管可通过模具法和静电纺丝法制备，但存在强度低、批次差异大等缺点，而编织法可以解决这个问题。在本研究中，聚乳酸纱线被用作编织纱线，并且编织过程中的纱锭数量变化以实现 4、5、6、7 和 8 个多通道人工神经导管。建立了满足多通道导管一定尺寸规格参数所需的编织纱锭数量的数学模型。通过扫描电子显微镜观察和力学测试对导管的横截面形态和力学性能进行了表征；结果表明，多通道结构构建良好；多通道导管的拉伸强度超过兔胫骨神经的 30 倍。对导管的生物相容性进行了测试；细胞培养结果证明编织多通道神经导管对施万细胞无毒，并且在多通道导管中，4 通道导管中的细胞粘附和增殖最佳，接近单通道导管。
