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侧柏叶提取物抗甲型流感病毒的作用。

The antiviral activity of Thuja orientalis folium against Influenza A virus.

机构信息

Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu, 41062 Republic of Korea.

Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu, 41062 Republic of Korea.

出版信息

Virus Res. 2023 Oct 2;335:199199. doi: 10.1016/j.virusres.2023.199199. Epub 2023 Aug 20.

DOI:10.1016/j.virusres.2023.199199
PMID:37582473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10445455/
Abstract

Thuja orientalis Folium (TOF) has been prescribed traditionally as an expectorant for inflammatory airway disease. In this study, we evaluated the anti-influenza A virus (IAV) activity of TOF by detecting GFP expressed by influenza A virus (A/PR/8/34-GFP) infection. The fluorescence microscopy and fluorescence-activated cell sorting analysis showed that TOF potently inhibited IAV infection, dose-dependently. Consistently, immunofluorescence and Q-PCR analysis results confirmed TOF significantly represses IAV protein and RNA expression. TOF inhibited IAV infection at the binding and entry step upon viral infection and interferes with HA protein. Further, TOF exhibited a virucidal effect and inhibited the neuraminidase activity of IAV. Additionally, TOF prevented the cytopathic effect caused by H1N1 and H3N2 IAV infection. Amentoflavone among the constituents in TOF exerted the strongest anti-IAV effect. Myricetin, quercetin, and quercitrin also inhibited IAV infection. However, the potent anti-IAV effect of TOF may be related to the synergistic effect of constituents, not by a single specific compound. Our results suggest TOF exhibits a significant inhibitory effect against IAV infection at multi-stages via the blockage of viral attachment and entry, inhibition of neuraminidase, and induction of virucidal effects.

摘要

云芝（Thuja orientalis Folium，TOF）传统上被用作治疗气道炎症性疾病的祛痰药。在这项研究中，我们通过检测流感 A 病毒（A/PR/8/34-GFP）感染表达的 GFP 来评估 TOF 的抗流感 A 病毒（IAV）活性。荧光显微镜和荧光激活细胞分选分析表明，TOF 能够强烈抑制 IAV 感染，呈剂量依赖性。一致地，免疫荧光和 Q-PCR 分析结果证实 TOF 显著抑制 IAV 蛋白和 RNA 表达。TOF 在病毒感染时抑制 IAV 感染的结合和进入步骤，并干扰 HA 蛋白。此外，TOF 表现出溶病毒作用并抑制 IAV 的神经氨酸酶活性。此外，TOF 可预防 H1N1 和 H3N2 IAV 感染引起的细胞病变效应。TOF 中的成分amentoflavone 表现出最强的抗 IAV 作用。杨梅素、槲皮素和槲皮苷也抑制 IAV 感染。然而，TOF 的强效抗 IAV 作用可能与成分的协同作用有关，而不是单一特定化合物的作用。我们的研究结果表明，TOF 通过阻断病毒附着和进入、抑制神经氨酸酶和诱导溶病毒作用，在多个阶段对 IAV 感染表现出显著的抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/cec0078c300a/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/57bcc1d11d06/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/e6a72b51407d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/8c757d69868f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/01a67c916474/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/361efb90b13b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/f295266ce265/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/21963a21e84e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/53533738f415/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/cec0078c300a/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/57bcc1d11d06/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/e6a72b51407d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/8c757d69868f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/01a67c916474/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/361efb90b13b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/f295266ce265/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/21963a21e84e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/53533738f415/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9c/10445455/cec0078c300a/gr9.jpg

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