Department of Biochemistry and Molecular Biology, Shantou University Medical College, 515041, Shantou, China.
Department of Central Laboratory, Shantou Central Hospital, 515041, Shantou, China.
Dokl Biochem Biophys. 2023 Jun;510(1):132-143. doi: 10.1134/S1607672922600269. Epub 2023 Aug 15.
LOX (Lysyl oxidase) family participates in the catalysis of collagen and elastin to maintain ECM homeostasis. Glioma is the most common primary brain tumor and LOX family has not been systemic studied in glioma. In this study, we found LOX family members are upregulated expressed in gliomas samples. A protein-protein interaction network (PPIN) was construct to visualize and understand the differential expression pattern, as well as functional annotation, for LOX family and their interacting proteins, which involved in collagen fibril organization and MAPK signaling pathway. Through subcellular localization distribution, the LOX family members distribute both intracellular and extracellular. All five LOX members are consistently significantly correlate with dendritic cell both in immune infiltrate of GBM and LGG. Survival analysis showed that high expression of LOX family is associated with a poor prognosis of gliomas patients. These analyses provide important clues to identify the potential biological roles for LOX family in gliomas, which might serve as diagnosis markers.
LOX（赖氨酰氧化酶）家族参与胶原蛋白和弹性蛋白的催化，以维持细胞外基质的动态平衡。神经胶质瘤是最常见的原发性脑肿瘤，LOX 家族在神经胶质瘤中的系统研究尚未进行。在这项研究中，我们发现 LOX 家族成员在神经胶质瘤样本中表达上调。构建蛋白质-蛋白质相互作用网络（PPIN）可视化和理解 LOX 家族及其相互作用蛋白的差异表达模式以及功能注释，这些蛋白参与胶原蛋白纤维组织和 MAPK 信号通路。通过亚细胞定位分布，LOX 家族成员分布在细胞内和细胞外。在 GBM 和 LGG 的免疫浸润中，所有五个 LOX 成员与树突状细胞均呈一致显著相关。生存分析表明，LOX 家族的高表达与神经胶质瘤患者的预后不良相关。这些分析为鉴定 LOX 家族在神经胶质瘤中的潜在生物学作用提供了重要线索，可能作为诊断标志物。
