Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Vet Parasitol Reg Stud Reports. 2020 Jan;19:100359. doi: 10.1016/j.vprsr.2019.100359. Epub 2019 Dec 4.
Fasciolosis is a zoonotic disease caused by Fasciola hepatica and Fasciola gigantica. Over the last decade, diagnostic tools to detect and differentiate Fasciola species have improved, but our knowledge of the distribution of haplotypes and gene flow of this parasite is not comprehensive yet. The purpose of this study was to investigate this gap in the epidemiology of F. hepatica in different provinces of Iran between 2015 and 2017. Isolated Fasciola were collected from abattoirs in 9 provinces. The partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene was used for the identification and molecular analysis of F. hepatica isolates. The amplified PCR products were purified and subjected to direct sequencing for subsequent construction of phylogenetic tree and network analysis. In the 130 subjects analyzed, 37 ND1 haplotypes were detected. This is the first study in Iran which investigates F. hepatica population and its genetic structure, based on mitochondrial ND1 marker in different geographical regions of Iran.
片形吸虫病是由肝片吸虫和巨片形吸虫引起的人畜共患疾病。在过去的十年中，用于检测和区分片形吸虫种的诊断工具已经得到了改进，但我们对该寄生虫的单倍型分布和基因流动的了解还不全面。本研究旨在调查 2015 年至 2017 年期间伊朗不同省份肝片形吸虫的流行病学中的这一差距。从 9 个省份的屠宰场采集分离的片形吸虫。使用线粒体 NADH 脱氢酶亚单位 1（ND1）基因的部分序列对肝片形吸虫分离株进行鉴定和分子分析。扩增的 PCR 产物经过纯化，直接测序，随后构建系统发育树和网络分析。在分析的 130 个样本中，检测到 37 种 ND1 单倍型。这是伊朗首次基于伊朗不同地理区域的线粒体 ND1 标记，对肝片形吸虫种群及其遗传结构进行的研究。
