Turan Ibrahim, Canbolat Deniz, Demir Selim, Kerimoglu Gokcen, Colak Fatih, Turkmen Alemdar Nihal, Mentese Ahmet, Aliyazicioglu Yüksel
Department of Biochemistry, Faculty of Pharmacy, University of Health Sciences, 06010, Ankara, Turkey.
Medicinal Aromatic Plants Application and Research Center, Artvin Coruh University, 08000, Artvin, Turkey.
Saudi Pharm J. 2023 Sep;31(9):101730. doi: 10.1016/j.jsps.2023.101730. Epub 2023 Aug 3.
Cisplatin (CDDP) is an important chemotherapeutic agent, accumulation of which in kidney tissue causes nephrotoxicity and renal failure. The aim of this study was to evaluate, for the first time in the literature, the protective effect of dimethyl sulfoxide (DMSO) extract of leaf (PVE) against CDDP-induced nephrotoxicity in rats. The PVE content was characterized using liquid chromatography-mass spectrometry. Nephrotoxicity was induced with a single dose of CDDP (7.5 mg/kg). Thirty female Wistar-Albino rats were divided into five groups (control, DMSO, CDDP (7.5 mg/kg), CDDP + PVE (25 mg/kg), and CDDP + PVE (50 mg/kg)). Biochemical and histopathological analyses were then performed. Rutin, gallic acid, -coumaric acid and protocatechuic acid were identified as major components of PVE. Total antioxidant status and glutathione (GSH) values increased significantly in the serum samples from the treatment group compared to the CDDP group, while blood urea nitrogen, creatinine, oxidative stress index, malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), total oxidant status, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) values decreased significantly. GSH levels increased significantly in the treatment group compared to the CDDP group, while TNF-α, caspase-3, 8-OHdG, MDA levels and damage scores decreased significantly. In conclusion, PVE exhibited strong protective effects through its anti-apoptotic, antioxidant, and anti-inflammatory activities against nephrotoxicity and oxidative damage caused by CDDP in rats.
顺铂（CDDP）是一种重要的化疗药物，其在肾组织中的蓄积会导致肾毒性和肾衰竭。本研究旨在首次在文献中评估紫叶紫薇叶二甲基亚砜（DMSO）提取物（PVE）对顺铂诱导的大鼠肾毒性的保护作用。采用液相色谱 - 质谱联用对PVE成分进行表征。用单剂量顺铂（7.5mg/kg）诱导肾毒性。将30只雌性Wistar - Albino大鼠分为五组（对照组、DMSO组、顺铂（7.5mg/kg）组、顺铂 + PVE（25mg/kg）组和顺铂 + PVE（50mg/kg）组）。然后进行生化和组织病理学分析。芦丁、没食子酸、对香豆酸和原儿茶酸被鉴定为PVE的主要成分。与顺铂组相比，治疗组血清样本中的总抗氧化状态和谷胱甘肽（GSH）值显著升高，而血尿素氮、肌酐、氧化应激指数、丙二醛（MDA）、肿瘤坏死因子 - α（TNF - α）、总氧化剂状态和8 - 羟基 - 2'-脱氧鸟苷（8 - OHdG）值显著降低。与顺铂组相比，治疗组GSH水平显著升高，而TNF - α、半胱天冬酶 - 3、8 - OHdG、MDA水平和损伤评分显著降低。总之，PVE通过其抗凋亡、抗氧化和抗炎活性对顺铂诱导的大鼠肾毒性和氧化损伤表现出强大的保护作用。
