Neubauer B L, Best K L, Hoover D M, Slisz M L, Van Frank R M, Goode R L
Fed Proc. 1986 Oct;45(11):2618-26.
Mesenchyme (UGM) and epithelium (UGE) isolated from the urogenital sinuses (UGS) of 17-day male and female rat embryos were separated by using a trypsinization procedure, grown on soft agar, transplanted into syngeneic pubertal male hosts as subcapsular renal grafts, and then collected after 29-30 days. Neither UGM nor UGE underwent prostatic morphogenesis when grown under these conditions. However, tissue recombinants composed of UGM + UGE grew and produced prostatic glands with acinar secretory material. Further, UGM + UGE recombinants were made by varying the proportions of mesenchymal and epithelial tissues. The size of the implants was a function of the absolute amount of mesenchyme; increasing the absolute amount of UGM produced larger specimens whereas varying the UGE had no effect. The UGM was also found to be essential for supporting the growth of small glandular elements derived from the ventral prostate of pubescent rats. Segments isolated from the terminal vesicles (TIPs) and from prostatic tissue adjacent to the urethra (PDCT) regressed when implanted alone under the kidney capsule. However, combination of the prostatic segments with UGM produced prostatic glands with relative wet weight and DNA content responses of the following order: UGM + TIP greater than UGM + PDCT = UGM + UGE. Two-dimensional gel electrophoretic protein patterns from UGM + PDCT and UGM + TIP specimens had differential expression of three protein regions unique to the ventral prostate Quantitative and qualitative responses of the TIP and PDCT segments to UGM inductive influences indicate that differences exist between the epithelia of the TIP and PDCT regions of the ventral lobes of the rat prostate.
从17日龄雄性和雌性大鼠胚胎的泌尿生殖窦（UGS）分离出的间充质（UGM）和上皮（UGE），通过胰蛋白酶消化程序进行分离，在软琼脂上培养，作为肾被膜下移植体移植到同基因青春期雄性宿主中，然后在29 - 30天后收集。在这些条件下培养时，UGM和UGE均未发生前列腺形态发生。然而，由UGM + UGE组成的组织重组体生长并产生了具有腺泡分泌物质的前列腺腺体。此外，通过改变间充质和上皮组织的比例制备了UGM + UGE重组体。植入物的大小是间充质绝对量的函数；增加UGM的绝对量会产生更大的标本，而改变UGE则没有影响。还发现UGM对于支持来自青春期大鼠腹侧前列腺的小腺泡成分的生长至关重要。从终末小泡（TIPs）和尿道相邻前列腺组织（PDCT）分离的片段单独植入肾被膜下时会退化。然而，前列腺片段与UGM组合产生的前列腺腺体，其相对湿重和DNA含量的反应顺序如下：UGM + TIP大于UGM + PDCT = UGM + UGE。来自UGM + PDCT和UGM + TIP标本的二维凝胶电泳蛋白质图谱显示，腹侧前列腺特有的三个蛋白质区域存在差异表达。TIP和PDCT片段对UGM诱导影响的定量和定性反应表明，大鼠前列腺腹叶TIP和PDCT区域的上皮之间存在差异。
