Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, USA.
Laryngoscope. 2024 Feb;134(2):779-785. doi: 10.1002/lary.30965. Epub 2023 Aug 16.
Systemic dehydration decreases total body blood volume; however, hemodynamic alterations at the level of local organs, such as the larynx, remain unclear. Here we sought to quantify superior thyroid artery (STA) blood flow after dehydration and rehydration using in vivo magnetic resonance angiography (MRA) and ultrasound imaging in a rat model.
Male Sprague-Dawley rats (N = 17) were included in this prospective, repeated measures design. Rats first underwent MRA to determine baseline STA cross-sectional area, followed by high-frequency in vivo ultrasound imaging to measure STA blood velocity at baseline. Next, rats were systemically dehydrated (water withholding), followed by rehydration (water ad-lib). Ultrasound imaging was repeated immediately after dehydration and following rehydration. The STA blood velocity and STA cross-sectional area were used to compute STA blood flow. Three rats served as temporal controls for ultrasound imaging. To determine if the challenges to hydration status affected the STA cross-sectional area, four rats underwent only MRA at baseline, dehydration, and rehydration.
Systemic dehydration resulted in 10.5% average body weight loss. Rehydration resulted in average body weight gain of 10.9%. Statistically significant reductions were observed in STA mean blood flow rate after dehydration. Rehydration reversed these changes to pre-dehydration levels. No significant differences were observed in STA cross-sectional area with dehydration or rehydration.
Systemic dehydration decreased blood flow in the superior thyroid artery. Rehydration restored blood flow in the STA. Change in hydration status did not alter the STA cross-sectional area. These preliminary findings demonstrate the feasibility of using ultrasound and MRA to quantify hemodynamic changes and visualize laryngeal blood vessels.
NA Laryngoscope, 134:779-785, 2024.
全身性脱水会减少全身血容量；然而，局部器官（如喉部）的血液动力学变化仍不清楚。本研究旨在通过活体磁共振血管造影（MRA）和超声成像技术，在大鼠模型中量化脱水和再水合后甲状腺上动脉（STA）的血流。
纳入雄性 Sprague-Dawley 大鼠（N=17）进行前瞻性重复测量设计。大鼠首先进行 MRA 以确定基线 STA 横截面积，然后进行高频体内超声成像以测量基线 STA 血流速度。接着，大鼠进行系统性脱水（禁水），然后再进行水合（自由饮水）。脱水后和再水合后立即重复进行超声成像。STA 血流速度和 STA 横截面积用于计算 STA 血流量。3 只大鼠作为超声成像的时间对照。为了确定水合状态的变化是否影响 STA 横截面积，4 只大鼠仅在基线、脱水和再水合时进行 MRA。
全身性脱水导致平均体重下降 10.5%。再水合导致平均体重增加 10.9%。脱水后 STA 平均血流率显著降低。再水合使这些变化恢复到脱水前的水平。脱水或再水合时，STA 横截面积无明显变化。
全身性脱水降低了甲状腺上动脉的血流。再水合恢复了 STA 的血流。水合状态的变化并未改变 STA 横截面积。这些初步发现表明，使用超声和 MRA 来量化血液动力学变化和可视化喉部血管是可行的。
无。喉科学，134：779-785，2024。
