Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, U.S.A.
Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A.
Laryngoscope. 2024 Nov;134(11):4636-4641. doi: 10.1002/lary.31631. Epub 2024 Jul 9.
Systemic dehydration may induce osmotic and oxidative stress in the vocal folds, but our knowledge of the biology and mitigation with rehydration is limited. The purpose of this experiment was to evaluate whether systemic dehydration induces vocal fold oxidative and osmotic stress and to compare the impact of rehydration by water intake versus electrolyte intake on osmotic and oxidative stress-related gene expression.
Four-month-old male Sprague-Dawley rats (N = 32) underwent water restriction. Rehydration was achieved with ad libitum access to water or electrolytes for 24 hours. Rats were divided into four groups: euhydration control, dehydration-only, dehydration followed by either water or electrolyte rehydration (n = 8/group). Gene expression was assessed via RT2 Gene Expression Profiler arrays.
With respect to oxidative stress, 10 genes were upregulated and 2 were downregulated after vocal fold dehydration compared with the euhydrated control. Concerning osmotic stress, six genes were upregulated with dehydration only, six genes were upregulated following rehydration with water, whereas a single gene was upregulated with electrolyte rehydration. All genes with significantly different expression between the rehydration groups showed lower expression with electrolytes compared with water.
The results support a potential role of oxidative and osmotic stresses in vocal folds related to systemic dehydration. The differences in stress-related gene expression in vocal fold tissue between rehydration with electrolytes or water, albeit modest, suggest that both rehydration options offer clinical utility to subjects experiencing vocal fold dehydration with preliminary evidence that electrolytes may be more effective than water in resolving osmotic stress.
NA (prospective animal study) Laryngoscope, 134:4636-4641, 2024.
全身性脱水可能会导致声带产生渗透和氧化应激，但我们对其生物学机制以及通过再水化进行缓解的了解有限。本实验旨在评估全身性脱水是否会导致声带产生氧化和渗透应激，并比较通过饮水和电解质摄入再水化对渗透和氧化应激相关基因表达的影响。
4 月龄雄性 Sprague-Dawley 大鼠（N=32）接受水限制。通过 24 小时自由摄入水或电解质来实现再水化。大鼠被分为四组：正常水合对照组、脱水组、脱水后再水化组（水或电解质）（n=8/组）。通过 RT2 基因表达谱分析评估基因表达。
与正常水合对照组相比，声带脱水后有 10 个基因上调，2 个基因下调，涉及氧化应激。涉及渗透应激的研究中，仅脱水时就有 6 个基因上调，水再水化时有 6 个基因上调，而电解质再水化时仅有 1 个基因上调。再水化组之间差异表达的所有基因在电解质组中的表达均低于水组。
这些结果支持氧化应激和渗透应激在与全身性脱水相关的声带中起潜在作用。电解质或水再水化在声带组织中与应激相关的基因表达存在差异，尽管差异较小，但这表明两种再水化选择都为经历声带脱水的患者提供了临床实用性，初步证据表明电解质可能比水更有效地解决渗透应激。
NA（前瞻性动物研究）《喉镜》，134：4636-4641，2024 年。
