College of Dental Medicine Illinois, Midwestern University, Downers Grove, Illinois, USA.
College of Graduate Studies, Midwestern University, Downers Grove, Illinois, USA.
Int Endod J. 2023 Nov;56(11):1337-1349. doi: 10.1111/iej.13962. Epub 2023 Aug 16.
Chitosan-based biomaterials exhibit several properties of biological interest for endodontic treatment. Herein, a low molecular weight chitosan (CH) solution was tested for its antimicrobial activity against Enterococcus faecalis (E. faecalis) and effects on dentine structure.
The root canal of 27 extracted uniradicular teeth were biomechanically prepared, inoculated with a suspension of E. faecalis and randomly assigned to be irrigated with either 5.25% sodium hypochlorite (NaClO), 0.2% CH or sterile ultrapure water (W). Bacteriologic samples were collected from root canals and quantified for of E. faecalis colony-forming units (CFUs). The effectiveness of CH over E. faecalis biofilms was further measured using the MBEC Assay®. Additionally, dentine beams and dentine powder were obtained, respectively, from crowns and roots of 20 extracted third molars. Dentine samples were treated or not with 17% EDTA and immersed in either CH or W for 1 min. The effects of CH on dentine structure were evaluated by assessment of the modulus of elasticity, endogenous proteolytic activity and biochemical modifications.
The number of E. faecalis CFUs was significantly lower for samples irrigated with CH and NaClO. No significant differences were found between CH and NaClO treatments. Higher modulus of elasticity and lower proteolytic activity were reported for dentine CH-treated specimens. Chemical interaction between CH and dentine was observed for samples treated or not with EDTA.
Present findings suggest that CH could be used as an irrigant during root canal treatment with the triple benefit of reducing bacterial activity, mechanically reinforcing dentine and inhibiting dentine proteolytic activity.
壳聚糖基生物材料具有多种生物学特性，可用于牙髓治疗。本研究旨在测试低分子量壳聚糖（CH）溶液对粪肠球菌（E. faecalis）的抗菌活性及其对牙本质结构的影响。
对 27 颗单根牙进行根管生物力学预备，接种粪肠球菌悬浮液，随机分为氯酸钠（NaClO）、0.2%CH 或无菌超纯水（W）冲洗组。从根管中采集细菌样本并定量检测粪肠球菌的菌落形成单位（CFU）。使用 MBEC 检测法进一步评估 CH 对粪肠球菌生物膜的效果。此外，从 20 颗第三磨牙的冠部和根部分别获得牙本质梁和牙本质粉。牙本质样本用或不用 17%EDTA 处理，分别浸入 CH 或 W 中 1 分钟。通过评估弹性模量、内源性蛋白水解活性和生化修饰来评估 CH 对牙本质结构的影响。
CH 和 NaClO 冲洗组粪肠球菌 CFU 数量明显减少。CH 和 NaClO 处理组之间无显著差异。CH 处理的牙本质样本弹性模量较高，蛋白水解活性较低。EDTA 处理或不处理的牙本质样本与 CH 之间存在化学相互作用。
本研究结果表明，CH 可用作根管冲洗液，具有减少细菌活性、机械增强牙本质和抑制牙本质蛋白水解活性三重功效。
