Department of Microbial Interactions, Friedrich Schiller University, Jena, Germany.
Cluster of Excellence Balance of the Microverse, Friedrich Schiller University, Jena, Germany.
Antonie Van Leeuwenhoek. 2023 Oct;116(10):995-1007. doi: 10.1007/s10482-023-01860-x. Epub 2023 Aug 16.
A novel planctomycetal strain, designated Pan189, was isolated from biofilm material sampled close to Panarea Island in the Tyrrhenian Sea. Cells of strain Pan189 are round grain rice-shaped, form pink colonies and display typical planctomycetal characteristics including asymmetric cell division through polar budding and presence of crateriform structures. Cells bear a stalk opposite to the division pole and fimbriae cover the cell surface. Strain Pan189 has a mesophilic (optimum at 24 °C) and neutrophilic (optimum at pH 7.5) growth profile, is aerobic and heterotrophic. Under laboratory-scale cultivation conditions, it reached a generation time of 102 h (µ = 0.0068 h), which places the strain among the slowest growing members of the phylum Planctomycetota characterized so far. The genome size of the strain is with 5.23 Mb at the lower limit among the family Planctomycetaceae (5.1-8.9 Mb). Phylogenetically, the strain represents a novel genus and species in the family Planctomycetaceae, order Planctomycetales, class Planctomycetia. We propose the name Stratiformator vulcanicus gen. nov., sp. nov. for the novel taxon, that is represented by the type strain Pan189 (= DSM 101711 = CECT 30699).
一株新的浮霉状菌菌株 Pan189 是从靠近意大利第勒尼安海的帕纳雷阿岛附近的生物膜材料中分离得到的。菌株 Pan189 的细胞呈圆形稻谷状，形成粉红色菌落，显示出典型的浮霉状菌特征，包括通过极生芽殖进行不对称细胞分裂和存在坑洼状结构。细胞在与分裂极相对的位置上带有一个柄，菌毛覆盖在细胞表面。菌株 Pan189 的生长特性为中温（最适温度为 24°C）和嗜中性（最适 pH 值为 7.5），是需氧异养菌。在实验室规模的培养条件下，其代时为 102 小时（µ = 0.0068 小时），这使该菌株成为迄今为止所描述的最缓慢生长的浮霉状菌门成员之一。该菌株的基因组大小为 5.23 Mb，是浮霉状菌科（5.1-8.9 Mb）中最小的。系统发育分析表明，该菌株代表了浮霉状菌科、浮霉状菌目、浮霉状菌纲中的一个新属和新种。我们提议将这个新的分类单元命名为 Stratiformator vulcanicus gen. nov., sp. nov.，代表菌株为 Pan189（= DSM 101711 = CECT 30699）。
