Kallscheuer Nicolai, Boedeker Christian, Wiegand Sandra, Kohn Timo, Heuer Anja, Overmann Jörg, Peters Stijn, Jogler Mareike, Rohde Manfred, Jogler Christian
Department of Microbial Interactions, Friedrich Schiller-University, Jena, Germany.
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Brunswick, Germany.
J Bacteriol. 2025 Jul 24;207(7):e0033724. doi: 10.1128/jb.00337-24. Epub 2025 Jun 24.
Members of the phylum are ubiquitous bacteria that play important roles in the global carbon and nitrogen cycle. In this study, we sampled the shallow-sea hydrothermal vent system close to Panarea Island, Italy, and analyzed the bacterial diversity in this habitat using a cultivation-independent amplicon sequencing approach. Motivated by the observed abundance of members of the phylum , we employed cultivation conditions that facilitate the enrichment of planctomycetes and isolated strain Pan216. This strain shows a rarely observed type of cell division-lateral budding. Based on 16S rRNA gene- and multi-locus sequence analyses, its phylogenetic position in the phylum appears vague. Strain Pan216 clustered between the different described families in the class . The novel isolate shares the highest sequence identity (85.4%) of the 16S rRNA gene with SVX8, indicating that this strain belongs to a novel family. In addition to its uncommon cell division mode, Pan216 cells are pill-shaped and covered by a putative outer surface layer. Genomic analyses of strain Pan216 revealed many giant genes, putative S-layer protein-encoding genes, and only a limited set of canonical cell division genes. Based on the results of the polyphasic analysis, we conclude that strain Pan216 constitutes a novel family within the phylum , for which we propose the name fam. nov. The novel species gen. nov., sp. nov. is represented by the type strain Pan216 (= DSM 100414 = CECT 9536).
We describe a novel family of the underrepresented bacterial phylum that divides by unusual lateral budding. Our strain is the only validly described species that uses this mode of cell division. Furthermore, it represents the only planctomycete outside of the anammox bacteria that has an S-layer-like structure. Taken together, the novel family shows a novel mechanism of cell division that could only be studied in this species.
[该门]的成员是普遍存在的细菌，在全球碳和氮循环中发挥着重要作用。在本研究中，我们对意大利帕纳雷阿岛附近的浅海热液喷口系统进行了采样，并使用非培养扩增子测序方法分析了该栖息地的细菌多样性。鉴于观察到的[该门]成员的丰度，我们采用了有利于浮霉菌富集的培养条件，并分离出了菌株Pan216。该菌株显示出一种罕见的细胞分裂类型——横向出芽。基于16S rRNA基因和多位点序列分析，其在[该门]中的系统发育位置似乎不明确。菌株Pan216聚集在[该纲]中不同描述的科之间。该新分离株与[某菌株]SVX8的16S rRNA基因具有最高的序列同一性（85.4%），表明该菌株属于一个新的科。除了其不寻常的细胞分裂模式外，Pan216细胞呈药丸状，并被假定的外表面层覆盖。对菌株Pan216的基因组分析揭示了许多巨大基因、假定的S层蛋白编码基因，以及一组有限的典型细胞分裂基因。基于多相分析的结果，我们得出结论，菌株Pan216构成了[该门]内的一个新科，我们将其命名为[新科名]。新物种[新属名] [新种名]由模式菌株Pan216（= DSM 100414 = CECT 9536）代表。
我们描述了一个代表性不足的细菌门的新科，该科通过不寻常的横向出芽进行分裂。我们的菌株是唯一有效描述的使用这种细胞分裂模式的物种。此外，它代表了除厌氧氨氧化细菌外唯一具有S层样结构的浮霉菌。综上所述，这个新科展示了一种只能在该物种中研究的新的细胞分裂机制。
