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使用共聚焦显微镜研究硅水凝胶接触镜的表面反射特性。

Study of silicone hydrogel contact lenses' surface reflection characteristics using confocal microscopy.

机构信息

Alcon Polska, Warsaw, Poland.

University of Warsaw, Warsaw, Poland.

出版信息

J Biomater Sci Polym Ed. 2023 Dec;34(17):2400-2410. doi: 10.1080/09205063.2023.2248816. Epub 2023 Sep 6.

DOI:10.1080/09205063.2023.2248816
PMID:37584778
Abstract

The physical and chemical properties of contact lenses (CLs) differ significantly from one another. This is already covered by the FDA classification, which divides soft lenses into groups and subgroups for additional characteristics. The differences relate to both the interior and surface of the lens. Several differences in the surface characteristics of individual contact lenses have been studied and demonstrated to date. However, one of their fundamental physical properties, that is light reflection or, quantitatively, reflectance has not been compared. This paper describes the surface differences of a range of silicone-hydrogel (SiHy) lenses using reflectance confocal microscopy. It shows the relationship between the amount of light reflected from the lens surface and the material parameters. Common SiHy lens materials were used in the study, including two lenses with surface modifications. Light incident at the interface between two media (phosphate-buffered saline and lens) with different refractive indices is partially reflected. The normalized results show significant differences between the reflection signals (1 vs 0.07), and that they are not correlated with the refractive index (R = 0.5536). For the water content (%HO), a general trend was observed that the higher the %HO, the lower the reflection signal is (R = 0.8105). The reflection signal and surface modulus show the best correlation. (R = 0.9883). The proposed CLs analysis method, using reflectance confocal microscopy, provides data to differentiate between lenses with and without surface modifications.

摘要

接触镜(CL)的物理和化学性质有很大差异。这已经涵盖在 FDA 分类中,该分类根据其他特性将软镜分为组和亚组。差异与镜片的内部和表面都有关。迄今为止,已经研究并证明了个别接触镜表面特性的一些差异。然而,它们的一个基本物理性质,即光反射或定量反射率尚未进行比较。本文使用反射共焦显微镜描述了一系列硅酮水凝胶(SiHy)镜片的表面差异。它显示了从镜片表面反射的光量与材料参数之间的关系。研究中使用了常见的 SiHy 镜片材料,包括两种具有表面改性的镜片。在两种具有不同折射率的介质(磷酸盐缓冲盐水和镜片)之间的界面处入射的光会部分反射。归一化结果显示反射信号之间存在显著差异(1 对 0.07),并且它们与折射率(R = 0.5536)无关。对于水含量(%HO),观察到一个总体趋势,即 %HO 越高,反射信号越低(R = 0.8105)。反射信号和表面模量显示出最佳相关性(R = 0.9883)。使用反射共焦显微镜的建议 CL 分析方法提供了区分具有和不具有表面改性的镜片的数据。

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