School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
Biofouling. 2021 Sep;37(8):862-878. doi: 10.1080/08927014.2021.1978433. Epub 2021 Oct 6.
The aim of this study was to develop an experimental methodology to measure lipid deposition with contact lenses. Contact lenses were incubated in a lipid solution. The amount and types of adsorbed lipids were assessed using mass spectrometry and confocal microscopy. The recovery of lipids from lenses varied with lipid and lens type. Most non-polar and polar lipids were desorbed from lenses during the first 5 min of extraction. Fluorescently labelled phosphatidylcholine bound within the matrix of Senofilcon A lenses but to the surface of Lotrafilcon B lenses, whereas fluorescently labelled cholesteryl ester was found throughout both lenses. The efficacy of extraction of lipids from contact lenses varies for different lipid classes and different lens materials. Differences in the amount and time of lipid desorption probably resulted from the strength of the bond between lipid and lens polymer and the depth of adsorption of lipid in the polymer.
本研究旨在开发一种实验方法来测量隐形眼镜中的脂质沉积。将隐形眼镜在脂质溶液中孵育。使用质谱和共焦显微镜评估吸附脂质的量和类型。从隐形眼镜中回收脂质的量因脂质和隐形眼镜类型而异。在提取的前 5 分钟内，大多数非极性和极性脂质从镜片中洗脱出来。荧光标记的磷脂结合在 Senofilcon A 镜片的基质内，但结合在 Lotrafilcon B 镜片的表面，而荧光标记的胆固醇酯则分布在两种镜片中。从隐形眼镜中提取不同脂质类别的脂质的效果因隐形眼镜材料而异。脂质洗脱量和时间的差异可能是由于脂质与镜片聚合物之间的键强度以及脂质在聚合物中的吸附深度不同所致。
