Department of Electronic Systems Engineering, Indian Institute of Science, Bangalore, India.
MS Ramaiah Memorial Hospital, Bangalore, India.
Biomed Microdevices. 2023 Aug 16;25(3):31. doi: 10.1007/s10544-023-00672-0.
Local field potentials, the extracellular electrical activities from brain regions, provide clinically relevant information about the status of neurophysiological conditions, including epilepsy. In this study, a 13-channel silicon-based single-shank microelectrode array (MEA) was designed and fabricated to record local field potentials (LFPs) from the different depths of a rat's brain. A titanium/gold layer was patterned as electrodes on an oxidized silicon substrate, and silicon dioxide was deposited as a passivation layer. The fabricated array was implanted in the somatosensory cortex of the right hemisphere of an anesthetized rat. The developed MEA was interfaced with an OpenBCI Cyton Daisy Biosensing Board to acquire the local field potentials. The LFPs were acquired at three different neurophysiological conditions, including baseline signals, chemically-induced epileptiform discharges, and recovered baseline signals after anti-epileptic drug (AED) administration. Further, time-frequency analyses were performed on the acquired biopotentials to study the difference in spatiotemporal features. The processed signals and time-frequency analyses clearly distinguish between pre-convulsant and post-AED baselines and evoked epileptiform discharges.
局部场电位是脑区的细胞外电活动，提供了与神经生理状态相关的临床信息，包括癫痫。在这项研究中，设计并制造了一个 13 通道硅基单轴微电极阵列 (MEA)，用于记录大鼠大脑不同深度的局部场电位 (LFP)。在氧化硅衬底上的钛/金层被图案化为电极，并且沉积二氧化硅作为钝化层。制造的阵列被植入麻醉大鼠右半球的体感皮层。开发的 MEA 与 OpenBCI Cyton Daisy 生物传感板接口，以获取局部场电位。在三种不同的神经生理条件下获取 LFPs，包括基线信号、化学诱导的癫痫样放电和抗癫痫药物 (AED) 给药后恢复的基线信号。此外，对获得的生物电位进行时频分析，以研究时空特征的差异。处理后的信号和时频分析清楚地区分了前惊厥和后 AED 基线以及诱发的癫痫样放电。
