Department of Cell Biology and Physiology, Brigham Young University; Neuroscience Center, Brigham Young University.
Department of Statistics, Brigham Young University.
J Vis Exp. 2024 Sep 27(211). doi: 10.3791/67065.
Complementary metal-oxide-semiconductor high-density microelectrode array (CMOS-HD-MEA) systems can record neurophysiological activity from cell cultures and ex vivo brain slices in unprecedented electrophysiological detail. CMOS-HD-MEAs were first optimized to record high-quality neuronal unit activity from cell cultures but have also been shown to produce quality data from acute retinal and cerebellar slices. Researchers have recently used CMOS-HD-MEAs to record local field potentials (LFPs) from acute, cortical rodent brain slices. One LFP of interest is seizure-like activity. While many users have produced brief, spontaneous epileptiform discharges using CMOS-HD-MEAs, few users reliably produce quality seizure-like activity. Many factors may contribute to this difficulty, including electrical noise, the inconsistent nature of producing seizure-like activity when using submerged recording chambers, and the limitation that 2D CMOS-MEA chips only record from the surface of the brain slice. The techniques detailed in this protocol should enable users to consistently induce and record high-quality seizure-like activity from acute brain slices with a CMOS-HD-MEA system. In addition, this protocol outlines the proper treatment of CMOS-HD-MEA chips, the management of solutions and brain slices during experimentation, and equipment maintenance.
互补金属氧化物半导体高密度微电极阵列 (CMOS-HD-MEA) 系统可以以前所未有的电生理细节记录细胞培养物和离体脑片的神经生理活动。CMOS-HD-MEAs 最初经过优化，可从细胞培养物中记录高质量的神经元单位活动，但也已被证明可从急性视网膜和小脑切片中产生高质量的数据。研究人员最近使用 CMOS-HD-MEAs 从急性皮质啮齿动物脑切片中记录局部场电位 (LFP)。一个感兴趣的 LFP 是癫痫样活动。虽然许多用户使用 CMOS-HD-MEAs 产生了短暂的自发性癫痫样放电，但很少有用户能够可靠地产生高质量的癫痫样活动。许多因素可能导致这种困难，包括电噪声、使用浸没式记录室产生癫痫样活动的不一致性质，以及二维 CMOS-MEA 芯片仅记录脑片表面的局限性。本方案中详述的技术应使用户能够使用 CMOS-HD-MEA 系统一致地诱导和记录高质量的急性脑切片癫痫样活动。此外，本方案概述了 CMOS-HD-MEA 芯片的正确处理、实验过程中溶液和脑片的管理以及设备维护。
