Department of Reproductive Medicine, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
Department of Reproductive medicine center, the Affiliated Weihai Second Municiple Hospital of Qingdao University, Weihai, Shandong, China.
Afr J Reprod Health. 2022 Jul;26(7):72-82. doi: 10.29063/ajrh2022/v26i7.8.
Cryopreservation, the most popular way to preserve human sperm, led to a significant decline in sperm motility. Here, we tried to introduce a new method to store sperm without freezing. Different concentrations of genistein were added to liquid preserved sperm. We investigated the effects of supplementation on sperm total antioxidative capacity (T-AOC), glutathione(GSH), methane dicarboxylic aldehyde (MDA), acrosomal enzyme activity, and fertilization ability of sperm. The effects of liquid storage and cryopreservation on sperm parameters were also compared. IVF medium supplemented with genistein (20µmol L-1) maintained sperm motility for up to 11 days. The addition of genistein led to a decrease in reactive oxygen species (ROS) generation that demonstrated an effective improvement in sperm motility and decreased the MDA production and maintained the GSH content and enhanced the oxidative stress resistance ability of the sperm during liquid storage. The storage sperm were used for intracytoplasmic sperm injection(ICSI) into human oocytes and activated oocytes successfully. Sperm stored in liquid medium containing genistein was superior to sperm stored in liquid nitrogen in terms of antioxidant stress and fertilization ability. We confirmed that genistein could be used as an antioxidant for the liquid storage of sperm. Sperm stored in an IVF medium with genistein could avoid cryodamage, which may become an alternative option in assisted reproduction technology.
冷冻保存是保存人类精子最常用的方法，但会导致精子活力显著下降。在这里，我们试图介绍一种新的不冷冻保存精子的方法。将不同浓度的染料木黄酮添加到液体保存的精子中。我们研究了补充剂对精子总抗氧化能力（T-AOC）、谷胱甘肽（GSH）、甲烷二羧酸醛（MDA）、顶体酶活性和精子受精能力的影响。还比较了液体储存和冷冻保存对精子参数的影响。含有染料木黄酮（20µmol L-1）的 IVF 培养基可将精子活力维持长达 11 天。添加染料木黄酮可减少活性氧（ROS）的产生，有效改善精子活力，降低 MDA 生成，保持 GSH 含量，并增强精子在液体储存过程中的抗氧化应激能力。将储存的精子用于人卵的胞浆内单精子注射（ICSI），并成功激活卵母细胞。与在液氮中储存的精子相比，含有染料木黄酮的液体培养基中储存的精子在抗氧化应激和受精能力方面具有优势。我们证实染料木黄酮可用作精子液体储存的抗氧化剂。在含有染料木黄酮的 IVF 培养基中储存的精子可以避免冷冻损伤，这可能成为辅助生殖技术的一种替代选择。
