IVF Centers Prof Zech, Bregenz, Austria.
Int J Vitam Nutr Res. 2012 Dec;82(6):391-8. doi: 10.1024/0300-9831/a000136.
This study aimed to investigate the influence of an oral antioxidative supplementation on sperm quality of in vitro fertilization (IVF) patients, as analyzed by sperm motility according to the WHO criteria and motile sperm organelle morphology examination (MSOME).
Semen samples were collected from 147 patients before undergoing an IVF/intracytoplasmic morphologically-selected sperm injection (IMSI) cycle and 2 - 12 months after an antioxidative supplementation. Semen analysis was evaluated according to WHO and MSOME criteria. Spermatozoa were grouped according to the size of nuclear vacuoles within the sperm's heads. Patients were divided into oligoasthenoteratozoospermic (OAT) and non-OAT men. Between first and second semen analysis, patients were supplemented orally with an antioxidative preparation.
After the antioxidative therapy we observed a significant reduction in the percentage of immotile sperm cells in the patients. Additionally, the percentage of class I spermatozoa according to MSOME criteria was significantly higher after antioxidative supplementation. In OAT patients the percentage of class I sperm was found to be increased, although not significantly. However, we observed a drastic improvement in sperm motility as well as in total sperm count in this group.
The results demonstrated a considerable improvement in semen quality, notably in OAT patients. Considering the putative relationship between semen quality on the one hand and reactive oxygen species on the other, the observed changes in the sperm parameters indicate that a decline in semen quality, and even subtle morphological changes, might be associated with oxidative stress. Our findings suggest that an antioxidative and micronutrient supplementation has a remarkable benefit for IVF patients having restricted sperm parameters, in particular.
本研究旨在通过世界卫生组织（WHO）标准和运动精子细胞器形态检查（MSOME）分析精子活力，探讨口服抗氧化补充剂对体外受精（IVF）患者精子质量的影响。
收集 147 例接受 IVF/胞浆内形态选择精子注射（IMSI）周期治疗的患者在治疗前和治疗后 2-12 个月的精液样本。根据 WHO 和 MSOME 标准进行精液分析。根据精子头部核空泡的大小将精子分为大小不同的核空泡组。患者分为少弱畸形精子症（OAT）和非 OAT 组。在第一次和第二次精液分析之间，患者口服抗氧化剂补充剂。
抗氧化治疗后，我们观察到患者不动精子的百分比显著降低。此外，根据 MSOME 标准，I 级精子的百分比在抗氧化补充后显著升高。在 OAT 患者中，I 级精子的百分比有所增加，但无统计学意义。然而，我们观察到该组的精子活力和总精子计数有明显改善。
结果表明，精液质量有了相当大的改善，尤其是在 OAT 患者中。考虑到精液质量一方面与活性氧之间的潜在关系，观察到的精子参数变化表明，精液质量下降甚至细微的形态变化可能与氧化应激有关。我们的研究结果表明，抗氧化和微量营养素补充对精子参数受限的 IVF 患者具有显著益处。
