Laboratory of Fungal Biology and Biotechnology, Institute of Pharmacy, Yerevan State University, 1 A. Manoogian St., 0025 Yerevan, Armenia.
Department of Analytical and Inorganic Chemistry, Yerevan State University, Armenia.
Int J Med Mushrooms. 2023;25(7):65-74. doi: 10.1615/IntJMedMushrooms.2023048520.
The qualitative analysis of hexane extracts obtained from different trama layers (WT, T1-T4) of dried fruiting bodies of medicinal bracket fungus Ganoderma applanatum collected in the Tavoush region of North-East Armenia was performed by GC-MS analysis. Three sterols [(7.22-ergostadienon, ergosterol and ergosta-14.22-diene-3-ol (3β, 5α, 22E)] have been identified. The results have shown that the content and ratio of sterols differ in analyzed trama samples. The highest amount of sterols was detected in middle parts of T2 and T3 layers, while content of sterols gradually decreased to the upper cortical (T4) and lower hymenial (T1) layers. The chromatographic profiles of identified compounds indicate that different sterols dominated in each layer: 7.22-ergostadienon in T4, ergosterol in T3, T2, and T1. The average weight loss of analyzed trama samples during six days of drying was about 40 wt.% (37.0-43.49 wt.%) of the total weight of basidiome, which decreased up to 5 wt.% in the next two days. The complete extraction of sterols lasted six days. Its further prolongation leads to stationary phase without an increase in the amount of extracted sterols.
采用 GC-MS 分析方法对采自亚美尼亚东北部塔武什地区药用担子菌层孔菌（Ganoderma applanatum）干燥子实体不同组织层（WT、T1-T4）的己烷提取物进行了定性分析。鉴定出三种甾醇[(7.22-麦角甾二烯醇、麦角甾醇和麦角甾-14.22-二烯-3-醇（3β，5α，22E)]。结果表明，分析的组织样品中甾醇的含量和比例不同。甾醇含量最高的部位是 T2 和 T3 层的中部，而甾醇含量逐渐减少到上部皮层（T4）和下部子实层（T1）。鉴定化合物的色谱图谱表明，不同的甾醇在每个层中占主导地位：T4 中的 7.22-麦角甾二烯醇、T3、T2 和 T1 中的麦角甾醇。分析的组织样品在六天的干燥过程中的平均失重约为担子菌总重量的 40wt.%（37.0-43.49wt.%），在接下来的两天内降至 5wt.%。甾醇的完全提取持续了六天。进一步延长其时间会导致达到固定阶段，而不会增加提取的甾醇的量。
