Doctoral School of Health Sciences, Faculty of Health Science, University of Pẻcs, Pẻcs, Hungary.
Hungarian University of Agriculture and Life Sciences, Institute of Horticultural Sciences, Budapest, Hungary.
Biomed Pharmacother. 2023 Oct;166:115325. doi: 10.1016/j.biopha.2023.115325. Epub 2023 Aug 14.
The evaluation of natural biomass sources is a promising strategy in accelerating the development of novel anti-cancer medications. Our study aimed to evaluate the activity of W. ugandensis ethanolic roots and stems extracts on the expression of five targeted genes (COX-2, CASPS-9, Bcl-xL, Bcl2 and 5-LOX) in colorectal cancer (CRC) cell lines (Caco-2). Plant extracts were obtained using serial exhaustive extraction and dissolved in Dimethyl sulfoxide appropriately for bioassay. Caco-2 cell lines were passaged, treated with plant extracts at varying concentrations and their RNA's isolated for evaluation. Our unique study reports on W. ugandensis as efficient natural inhibitors of CRC growth, by directly linking its phytoconstituents to; downregulation of COX-2, 5-LOX, Bcl-xL, Bcl2 and upregulation of CASPS9 genes dose-dependently. We present W. ugandensis ethanolic roots and stems extracts as promising natural inhibitors for CRC carcinogenesis and recommend in vivo and subsequent clinical trials, with substantial clinical effects postulated. We further suggest studies on identification and characterization of the specific metabolites in W. ugandensis involved in the modulatory mechanisms, resulting to inhibition of CRC growth and possible metastases.
评估天然生物质资源是加速新型抗癌药物开发的有前途的策略。我们的研究旨在评估 W. ugandensis 乙醇根和茎提取物对结直肠癌细胞系（Caco-2）中五个靶向基因（COX-2、CASPS-9、Bcl-xL、Bcl2 和 5-LOX）表达的活性。使用连续的彻底提取法获得植物提取物，并在适当的二甲基亚砜中溶解以供生物测定。传代 Caco-2 细胞系，用不同浓度的植物提取物处理，并分离其 RNA 进行评估。我们的独特研究报告称，W. ugandensis 是 CRC 生长的有效天然抑制剂，通过直接将其植物成分与 COX-2、5-LOX、Bcl-xL、Bcl2 的下调和 CASPS9 基因的上调相关联，呈剂量依赖性。我们提出 W. ugandensis 乙醇根和茎提取物作为 CRC 癌变的有前途的天然抑制剂，并建议进行体内和随后的临床试验，预计会有显著的临床效果。我们进一步建议对 W. ugandensis 中参与调节机制的特定代谢物进行鉴定和表征的研究，从而抑制 CRC 的生长和可能的转移。
