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：甲醇提取物在结直肠癌中的抗肿瘤活性及基因调控

Antitumor Activity of : Methanolic Extracts and Gene Regulation in Colorectal Cancer.

作者信息

Macharia John M, Maina John K, Zand Afshin, Rono Cheriro Betsy, Varjas Tímea, Sipos Dávid, Káposztás Zsolt, Budán Ferenc, Kövesdi Orsolya Liza, Raposa Bence L

机构信息

Doctoral School of Health Sciences, Faculty of Health Science, University of Pẻcs, 7621 Pécs, Hungary.

Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary.

出版信息

Nutrients. 2025 Jan 28;17(3):471. doi: 10.3390/nu17030471.

DOI:10.3390/nu17030471
PMID:39940328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11819982/
Abstract

A promising approach to accelerating the development of innovative anti-cancer therapies involves the evaluation of natural plant compounds. In this study, we focused on examining the effects of () methanolic root and stem infusions on the activity of five target genes-, , , , and -using colorectal cancer (CRC) cell lines (Caco-2). The plant extracts were prepared for testing by dissolving them in dimethyl sulfoxide (DMSO) after undergoing a step-by-step extraction process. Caco-2 cells were then treated with different concentrations of the extracts, and RNA was extracted and purified for analysis. Our results demonstrated a dose-dependent relationship between the phytoconstituents of and the overexpression of , along with the downregulation of , , , and genes. This suggests that acts as a potent natural inhibitor of CRC progression. Given the potential clinical benefits, we propose the use of methanolic root and stem extracts as promising organic inhibitors for CRC tumorigenesis, with more in vitro studies warranted to validate and expand on our findings. Additionally, we recommend further studies to identify and characterize the specific metabolites in that contribute to the modulation of pathways responsible for inhibiting CRC growth.

摘要

一种加速创新抗癌疗法开发的有前景的方法涉及对天然植物化合物的评估。在本研究中，我们聚焦于使用结直肠癌（CRC）细胞系（Caco-2）来检测（）甲醇根和茎浸液对五个靶基因——、、、和——活性的影响。在经过逐步提取过程后，将植物提取物溶解于二甲基亚砜（DMSO）中制备用于测试。然后用不同浓度的提取物处理Caco-2细胞，并提取和纯化RNA用于分析。我们的结果表明，的植物成分与的过表达之间存在剂量依赖关系，同时、、和基因下调。这表明作为CRC进展的一种有效天然抑制剂。鉴于潜在的临床益处，我们建议使用甲醇根和茎提取物作为CRC肿瘤发生的有前景的有机抑制剂，需要更多的体外研究来验证和扩展我们的发现。此外，我们建议进一步研究以鉴定和表征中有助于调节负责抑制CRC生长的途径的特定代谢物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/dd52c920774a/nutrients-17-00471-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/437a04d0b280/nutrients-17-00471-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/d42a543341a3/nutrients-17-00471-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/18f9d9251af8/nutrients-17-00471-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/8b9f0fe0fa7e/nutrients-17-00471-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/edb9cd13c7d7/nutrients-17-00471-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/dd52c920774a/nutrients-17-00471-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/437a04d0b280/nutrients-17-00471-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/d42a543341a3/nutrients-17-00471-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/18f9d9251af8/nutrients-17-00471-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/8b9f0fe0fa7e/nutrients-17-00471-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/edb9cd13c7d7/nutrients-17-00471-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0ef/11819982/dd52c920774a/nutrients-17-00471-g006.jpg

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本文引用的文献

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Biomed Pharmacother. 2023 Oct;166:115325. doi: 10.1016/j.biopha.2023.115325. Epub 2023 Aug 14.
2
Medicinal Characteristics of L. in Colorectal Cancer Management.L.在结直肠癌治疗中的药用特性。
Pharmaceuticals (Basel). 2023 Jun 22;16(7):915. doi: 10.3390/ph16070915.
3
Pharmacotherapeutic Potential of against Colorectal Cancer Growth and Proliferation.
[具体药物名称]对结直肠癌生长和增殖的药物治疗潜力。 （你原文中“against”前少了具体药物名称，我按格式翻译了，你可补充完整药物名后追问我更准确的内容）
Pharmaceutics. 2023 May 22;15(5):1558. doi: 10.3390/pharmaceutics15051558.
4
Targeted lactate dehydrogenase genes silencing in probiotic lactic acid bacteria: A possible paradigm shift in colorectal cancer treatment?靶向乳酸脱氢酶基因沉默在益生菌乳杆菌中：结直肠癌治疗的一种可能范式转变？
Biomed Pharmacother. 2023 Apr;160:114371. doi: 10.1016/j.biopha.2023.114371. Epub 2023 Feb 7.
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Pharmacological effects and mechanisms of tannic acid.鞣酸的药理作用及作用机制。
Biomed Pharmacother. 2022 Oct;154:113561. doi: 10.1016/j.biopha.2022.113561. Epub 2022 Aug 24.
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Medicinal plants with anti-colorectal cancer bioactive compounds: Potential game-changers in colorectal cancer management.具有抗结直肠癌生物活性化合物的药用植物：结直肠癌管理中的潜在变革者。
Biomed Pharmacother. 2022 Sep;153:113383. doi: 10.1016/j.biopha.2022.113383. Epub 2022 Jul 9.
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