Macharia John M, Varjas Timea, Mwangi Ruth W, Káposztás Zsolt, Rozmann Nóra, Pintér Márton, Wagara Isabel N, Raposa Bence L
Doctoral School of Health Sciences, Faculty of Health Science, University of Pẻcs, 7621 Pẻcs, Hungary.
Department of Public Health Medicine, Medical School, University of Pẻcs, 7621 Pẻcs, Hungary.
Cancers (Basel). 2023 Oct 16;15(20):5002. doi: 10.3390/cancers15205002.
Colon tumors have a very complicated and poorly understood pathogenesis. Plant-based organic compounds might provide a novel source for cancer treatment with a sufficient novel mode of action. The objective of this study was to analyze and evaluate the efficacy of (AS) methanolic extracts on the expression of , , , , and in colorectal cancer (CRC) management. Caco-2 cell lines were used in the experimental study. In the serial exhaustive extraction (SEE) method, methanol was utilized as the extraction solvent. Upon treatment of with the methanolic extracts, the expression of the genes was progressively upregulated, thus, dose-dependently increasing the rate of apoptosis. On the other hand, the expressions of , , and were variably downregulated in a dose-dependent manner. This is a unique novel study that evaluated the effects of AS methanolic extracts in vitro on CRC cell lines using different dosage concentrations. We, therefore, recommend the utilization of AS and the application of methanol as the extraction solvent of choice for maximum modulatory benefits in CRC management. In addition, we suggest research on the specific metabolites in AS involved in the modulatory pathways that suppress the development of CRC and potential metastases.
结肠肿瘤的发病机制非常复杂，目前仍知之甚少。植物性有机化合物可能为癌症治疗提供一种具有充分新颖作用方式的新来源。本研究的目的是分析和评估（AS）甲醇提取物在结直肠癌（CRC）治疗中对、、、和表达的疗效。实验研究使用了Caco-2细胞系。在连续 exhaustive 提取（SEE）方法中，甲醇被用作提取溶剂。用甲醇提取物处理后，这些基因的表达逐渐上调，从而剂量依赖性地增加凋亡率。另一方面，、和的表达以剂量依赖性方式不同程度地下调。这是一项独特的新研究，使用不同剂量浓度在体外评估了AS甲醇提取物对CRC细胞系的影响。因此，我们建议使用AS，并将甲醇作为首选提取溶剂，以在CRC治疗中获得最大的调节益处。此外，我们建议对AS中参与抑制CRC发展和潜在转移的调节途径的特定代谢物进行研究。
