Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
J Nutr. 2023 Oct;153(10):3131-3143. doi: 10.1016/j.tjnut.2023.08.010. Epub 2023 Aug 14.
Obesity is associated with increased intestinal permeability and a diminished immune response. Phosphatidylcholine (PC), a form of choline found in eggs, has been shown to beneficially modulate T-cell response in the context of obesity when provided as the sole form of choline in the diet.
This study aimed to determine the impact of varying doses of PC as part of a high-fat diet (HFD) on immune cell function and intestinal permeability.
Male Wistar rats 4 wk of age were randomly assigned to consume 1 of 6 diets for 12 wk containing the same amount of total choline but differing in the forms of choline: 1-control low-fat (CLF, 20% fat, 100% free choline [FC]); 2-control high-fat (CHF, 50% fat, 100% FC); 3-100% PC (100PC, 50% fat, 100% egg-PC); 4-75% PC (75PC, 50% fat, 75% egg-PC+25% FC); 5-50% PC (50PC, 50% fat, 50% egg-PC+50% FC); and 6-25% PC (25PC; 50% fat, 25% egg-PC+75% FC). Intestinal permeability was measured by fluorescein isothiocyanate-dextran. Immune function was assessed by ex vivo cytokine production of splenocytes and cells isolated from the mesenteric lymph node (MLN) after stimulation with different mitogens.
Feeding the CHF diet increased intestinal permeability compared with the CLF diet, and doses of PC 50% or greater returned permeability to levels similar to that of the CLF diet. Feeding the CHF diet lowered splenocyte production of interleukin (IL)-1β, IL-2, IL-10, and tumor necrosis factor-alpha, and MLN production of IL-2 compared with the CLF group. The 50PC diet most consistently significantly improved cytokine levels (IL-2, IL-10, tumor necrosis factor-alpha) compared with the CHF diet.
Our results show that a dose of 50% of total choline derived from egg-PC can ameliorate HFD-induced intestinal permeability and immune cell dysfunction.
肥胖与肠道通透性增加和免疫反应减弱有关。当作为饮食中胆碱的唯一形式提供时，磷脂酰胆碱（PC），一种存在于鸡蛋中的胆碱形式，已被证明可以有益地调节肥胖情况下的 T 细胞反应。
本研究旨在确定作为高脂肪饮食（HFD）一部分的不同剂量 PC 对免疫细胞功能和肠道通透性的影响。
4 周龄雄性 Wistar 大鼠随机分为 6 组中的 1 组，在 12 周内食用含有相同总量胆碱但胆碱形式不同的 6 种饮食之一：1-对照低脂（CLF，20%脂肪，100%游离胆碱[FC]）；2-对照高脂（CHF，50%脂肪，100% FC）；3-100% PC（100PC，50%脂肪，100% 鸡蛋-PC）；4-75% PC（75PC，50%脂肪，75% 鸡蛋-PC+25% FC）；5-50% PC（50PC，50%脂肪，50% 鸡蛋-PC+50% FC）；和 6-25% PC（25PC；50%脂肪，25% 鸡蛋-PC+75% FC）。通过荧光素异硫氰酸酯-葡聚糖测量肠道通透性。通过用不同有丝分裂原刺激后分离的脾细胞和肠系膜淋巴结（MLN）中的细胞的细胞因子产生来评估免疫功能。
喂养 CHF 饮食会增加肠道通透性，而 PC 剂量为 50%或更高会使通透性恢复到与 CLF 饮食相似的水平。喂养 CHF 饮食会降低脾细胞产生白细胞介素（IL）-1β、IL-2、IL-10 和肿瘤坏死因子-α，以及 MLN 产生 IL-2 与 CLF 组相比。与 CHF 饮食相比，50PC 饮食最一致地显著改善了细胞因子水平（IL-2、IL-10、肿瘤坏死因子-α）。
我们的结果表明，源自鸡蛋-PC 的总胆碱 50%的剂量可以改善 HFD 诱导的肠道通透性和免疫细胞功能障碍。
