Lewis Erin D, Richard Caroline, Goruk Susan, Wadge Emily, Curtis Jonathan M, Jacobs René L, Field Catherine J
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Jean Mayer USDA Human Nutrition Center on Aging, Tufts University, Boston, MA 02111, USA.
Nutrients. 2017 Jul 7;9(7):713. doi: 10.3390/nu9070713.
Study objectives were to examine the impact of feeding a mixture of choline forms, or a diet high in glycerophosphocholine (GPC) on maternal immune function and offspring growth during lactation. Lactating Sprague-Dawley rat dams ( = 6/diet) were randomized to one of three diets, providing 1 g/kg total choline: Control (100% free choline (FC)), Mixed Choline (MC; 50% phosphatidylcholine (PC), 25% FC, 25% GPC), or High GPC (HGPC; 75% GPC, 12.5% PC, 12.5% FC). At 3 weeks, cell phenotypes and cytokine production with Concanavalin A (ConA)-or lipopolysaccharide (LPS)-stimulated splenocytes and mesenteric lymphocytes were measured. Feeding MC or HGPC diets improved pups' growth compared to Control (+22% body weight, < 0.05). In spleen, MC-and HGPC-fed dams had higher proportions of cytotoxic (CD8+) T cells expressing CD27, CD71 and CD127, total B cells (CD45RA+) and dendritic cells (OX6+OX62+), and produced less IL-6 and IFN-γ after ConA than Control-fed dams ( < 0.05). MC and HGPC LPS-stimulated splenocytes produced less IL-1β and IL-6 than Control. ConA-stimulated mesenteric lymphocytes from MC and HGPC dams produced more IL-2 and IFN-γ than Control ( < 0.05). In summary, feeding a mixture of choline forms during lactation improved offspring growth and resulted in a more efficient maternal immune response following mitogenic immune challenge.
研究目的是检验在哺乳期饲喂胆碱形式混合物或富含甘油磷酸胆碱（GPC）的日粮对母体免疫功能和后代生长的影响。将哺乳期的斯普拉格-道利大鼠母鼠（每组 = 6只）随机分为三种日粮组之一，总胆碱含量为1 g/kg：对照组（100%游离胆碱（FC））、混合胆碱组（MC；50%磷脂酰胆碱（PC）、25% FC、25% GPC）或高GPC组（HGPC；75% GPC、12.5% PC、12.5% FC）。在第3周时，测量用伴刀豆球蛋白A（ConA）或脂多糖（LPS）刺激的脾细胞和肠系膜淋巴细胞的细胞表型和细胞因子产生情况。与对照组相比，饲喂MC或HGPC日粮可改善幼崽生长（体重增加22%，P < 0.05）。在脾脏中，与对照组相比，饲喂MC和HGPC的母鼠中表达CD27、CD71和CD127的细胞毒性（CD8 +）T细胞、总B细胞（CD45RA +）和树突状细胞（OX6 + OX62 +）的比例更高，ConA刺激后产生的IL-6和IFN-γ更少（P < 0.05）。MC和HGPC刺激的LPS脾细胞产生的IL-1β和IL-6比对照组少。来自MC和HGPC母鼠的ConA刺激的肠系膜淋巴细胞产生的IL-2和IFN-γ比对照组多（P < 0.05）。总之，哺乳期饲喂胆碱形式混合物可改善后代生长，并在有丝分裂免疫挑战后导致更有效的母体免疫反应。
