China Tobacco Jiangsu Industrial Co., Ltd., Nanjing, 210000, Jiangsu, China.
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, Henan, China.
Sci Rep. 2023 Aug 16;13(1):13333. doi: 10.1038/s41598-023-40502-0.
During the processing of tobacco leaves, flue-curing and redrying can affect the structure of bacterial community, having an effect on the aging quality of tobacco leaves. In order to characterize the effects of flue-curing and redrying on the bacterial community of tobacco leaves, the bacterial community of samples at different processing stages (before flue-curing, after flue-curing, before redrying and after redrying) was analyzed using Illumina sequencing. A total of 33 phyla, 79 classes, 195 orders, 344 families, 826 genera and 7922 ASVs were obtained from 36 samples. There was no significant difference in the core bacterial groups of tobacco leaf at four processing stages. Proteobacteria dominated at the phylum level. Sphingomonas, Pseudomonas and Methylobacterium were the main genera shared by all samples. The functional prediction by PICRUSt showed an increase in the relative abundance of pathway related to metabolism after flue-curing and pathway related to environmental information processing after redrying. This study, we analyzed the changes of bacterial community and structural composition of tobacco leaves from flue-curing to redrying, and found that flue-curing had a greater effect on the microbial community than redrying. This is conducive for the exploration of microbial resources and improvement of tobacco leaf quality.
在烟叶加工过程中，烘烤和复烤会影响细菌群落的结构，从而影响烟叶的陈化品质。为了研究烘烤和复烤对烟叶细菌群落的影响，本研究采用 Illumina 测序技术对不同加工阶段（烘烤前、烘烤后、复烤前和复烤后）的样品中的细菌群落进行了分析。从 36 个样本中共获得了 33 个门、79 个纲、195 个目、344 个科、826 个属和 7922 个 ASVs。四个加工阶段烟叶的核心细菌群没有显著差异。在门水平上，变形菌门占优势。所有样品共有的主要属是鞘氨醇单胞菌属、假单胞菌属和甲基杆菌属。PICRUSt 功能预测表明，烘烤后与代谢相关的途径和复烤后与环境信息处理相关的途径的相对丰度增加。本研究分析了从烘烤到复烤烟叶细菌群落的变化和结构组成，发现烘烤对微生物群落的影响大于复烤。这有助于探索微生物资源和提高烟叶质量。
