Department of Infectious Disease Control and Prevention, Hangzhou Center of Disease Control and Prevention, Hangzhou, China.
Curr Microbiol. 2023 Aug 16;80(10):321. doi: 10.1007/s00284-023-03436-4.
Many fruits are consumed raw and act as vehicles for spreading antibiotic-resistant bacteria to consumers; hence, preventing foodborne diseases and ensuring food safety of fresh fruits are challenging. In this study, we aimed to analyze contamination in fruits and characterize antibiotic resistance genes in pathogenic microorganisms isolated from fruits. Sixty fruit samples were collected and screened for pathogenic microorganisms. The strains were identified, and the minimum inhibitory concentration for various antibiotics was determined. Antibiotic-resistant strains were analyzed by whole-genome sequencing. We isolated strain L46 from lemon samples and identified it as Rahnella aquatilis using MALDI-TOF MS and 16S rRNA sequencing. The whole genome of R. aquatilis L46 was 4.94 Mb and contained 291 putative antibiotic resistance genes (6.53%), including the gene coding for β-lactamase RAHN-1 CTX-M-2 and conferring resistance to ampicillin, polymyxin B, nitrofurantoin, imipenem, aztreonam, and cefotaxime. Thus, fruits can pose a potential hazard to human health and require greater surveillance and attention, as they may contain pathogenic and multidrug-resistant bacteria.
许多水果都是生食的，它们是将抗生素耐药菌传播给消费者的载体；因此，预防食源性疾病和确保新鲜水果的食品安全是具有挑战性的。在本研究中，我们旨在分析水果中的污染情况，并对从水果中分离出的病原菌的抗生素耐药基因进行特征分析。采集了 60 个水果样本并对其进行了病原菌筛选。对分离出的菌株进行鉴定，并测定了各种抗生素的最小抑菌浓度。对耐药菌株进行全基因组测序分析。我们从柠檬样本中分离出菌株 L46，并通过 MALDI-TOF MS 和 16S rRNA 测序将其鉴定为 Aquatilis 属的拉恩氏菌。Aquatilis 属 L46 的全基因组为 4.94 Mb，包含 291 个潜在的抗生素耐药基因（6.53%），其中包括编码β-内酰胺酶 RAHN-1 CTX-M-2 的基因，该基因对氨苄西林、多黏菌素 B、呋喃妥因、亚胺培南、氨曲南和头孢噻肟具有耐药性。因此，水果可能对人类健康构成潜在威胁，需要加强监测和关注，因为它们可能含有致病和多药耐药菌。
