Xu Shuai, Zhao Yurong, Peng Yue, Shi Yanxia, Xie Xuewen, Chai Ali, Li Baoju, Li Lei
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2022 Apr 4;13:850084. doi: 10.3389/fmicb.2022.850084. eCollection 2022.
Many strains have been widely described as plant growth-promoting rhizobacteria with the potential to benefit plant growth and protect plants from pathogens. ZF458 is a beneficial plant bacterium isolated from swamp soil with the potential for biocontrol. Strain ZF458 has shown broad-spectrum antagonistic activities against a variety of plant pathogens and exhibited a dramatic effect on controlling in sunflowers. The ZF458 genome sequence contained a 4,861,340-bp circular chromosome and two plasmids, with an average G + C content of 52.20%. Phylogenetic analysis demonstrated that ZF458 was closely related to SAP-19. Genome annotation and comparative genomics identified the conservation and specificity of large numbers of genes associated with nitrogen fixation, plant growth hormone production, organic acid biosynthesis and pyrroloquinoline quinone production that specific to benefiting plants in strain ZF458. In addition, numerous conserved genes associated with environmental adaption, including the bacterial secretion system, selenium metabolism, two-component system, flagella biosynthesis, chemotaxis, and acid resistance, were also identified in the ZF458 genome. Overall, this was the first study to systematically analyze the genes linked with plant growth promotion and environmental adaption in . The aim of this study was to derive genomic information that would provide an in-depth insight of the mechanisms of plant growth-promoting rhizobacteria, and could be further exploited to improve the application of ZF458 in the agriculture field.
许多菌株已被广泛描述为具有促进植物生长潜力并能保护植物免受病原体侵害的植物促生根际细菌。ZF458是一种从沼泽土壤中分离出的具有生物防治潜力的有益植物细菌。菌株ZF458已显示出对多种植物病原体的广谱拮抗活性，并在控制向日葵病害方面表现出显著效果。ZF458基因组序列包含一个4,861,340碱基对的环状染色体和两个质粒，平均G + C含量为52.20%。系统发育分析表明ZF458与SAP - 19密切相关。基因组注释和比较基因组学确定了大量与固氮、植物生长激素产生、有机酸生物合成和吡咯喹啉醌产生相关的基因在菌株ZF458中对植物有益的保守性和特异性。此外，在ZF458基因组中还鉴定出许多与环境适应相关的保守基因，包括细菌分泌系统、硒代谢、双组分系统、鞭毛生物合成、趋化性和耐酸性。总体而言，这是首次对与植物生长促进和环境适应相关基因进行系统分析的研究。本研究的目的是获取基因组信息，以深入了解植物促生根际细菌的作用机制，并可进一步用于改进ZF458在农业领域的应用。
