Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
J Agric Food Chem. 2023 Aug 30;71(34):12741-12748. doi: 10.1021/acs.jafc.3c04617. Epub 2023 Aug 16.
Increased drug resistance has significantly reduced the effectiveness of antibiotics used in the treatment of . Therefore, there has been a trend toward the development of new antimicrobial agents to circumvent drug resistance. In this study, we isolated and purified a novel bacteriocin, HA2-5, from HA2, which effectively killed through membrane disruption at a minimum inhibitory concentration (MIC) of 8 μg/mL. HA2-5 with 2× MIC was able to kill 99.9% of within 24 h. HA2-5 shows excellent stability and tolerance to temperature, pH, proteases, chemical reagents, UV radiation, and metal ions, with almost no loss of inhibitory activity after treatment. In addition, the very low hemolytic activity and cytotoxicity suggest that HA2-5 is biosafe. Notably, HA2-5 exhibits preferred antibacterial activity against gram-positive pathogens with an MIC of 16-32 μg/mL. In conclusion, this study shows that bacteriocin HA2-5 has the potential to be used as an alternative to antibiotics for acne treatment.
抗生素耐药性的增加显著降低了用于治疗痤疮的抗生素的疗效。因此，人们一直致力于开发新的抗菌药物来规避耐药性。在这项研究中，我们从痤疮丙酸杆菌 HA2 中分离和纯化了一种新型细菌素 HA2-5，其最低抑菌浓度（MIC）为 8 μg/mL 时即可有效杀伤痤疮丙酸杆菌。HA2-5 的 2×MIC 可在 24 h 内杀死 99.9%的痤疮丙酸杆菌。HA2-5 表现出良好的稳定性和对温度、pH 值、蛋白酶、化学试剂、UV 辐射和金属离子的耐受性，处理后几乎没有抑制活性损失。此外，HA2-5 的溶血活性和细胞毒性非常低，表明其生物安全性良好。值得注意的是，HA2-5 对革兰氏阳性病原体表现出优先的抗菌活性，其 MIC 值为 16-32 μg/mL。总之，这项研究表明细菌素 HA2-5 有可能替代抗生素用于痤疮治疗。
