Department of Biophysics, Institute of Quantum Biology, Sungkyunkwan University, Suwon, 16419, South Korea.
Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon, 16419, South Korea.
Adv Sci (Weinh). 2023 Oct;10(28):e2302072. doi: 10.1002/advs.202302072. Epub 2023 Aug 16.
The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.
新冠疫情爆发引发了公共卫生和全球卫生危机。然而，现场缺乏快速、可靠、敏感和低成本的逆转录聚合酶链反应（RT-PCR）检测限制了早期检测、及时隔离以及疫情防控。在这里，作者报告了一种通过芯片上 RT-定量 PCR（RT-qPCR）实现的快速移动传染病诊断方法：MEDIC-PCR。首先，作者使用卷对卷印刷工艺制造了基于廉价碳黑的一次性 PCR 芯片，可实现快速 LED 诱导光热 PCR 循环。MEDIC-PCR 可完成 RT（3 分钟）和 PCR（9 分钟）步骤。此外，作者对 89 名新冠患者和 103 名非新冠患者进行了检测，结果显示 MEDIC-PCR 具有出色的诊断准确性（97%）、灵敏度（94%）和特异性（98%）。这种 MEDIC-PCR 可以为未来的大流行提供全球预防性健康服务。
