Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea.
33, Sagimakgol-ro 62beon-gil, Jungwon-gu, Seongnam, 13211, Republic of Korea.
Anal Bioanal Chem. 2022 Jul;414(16):4685-4696. doi: 10.1007/s00216-022-04090-8. Epub 2022 May 2.
Respiratory illness caused by influenza virus is a serious public health problem worldwide. As the symptoms of influenza virus infection are similar to those of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, it is essential to distinguish these two viruses. Therefore, to properly respond to a pathogen, a detection method that is capable of rapid and accurate diagnosis in a hospital or at home is required. To satisfy this need, we applied loop-mediated isothermal amplification (LAMP) and an isothermal nucleic acid amplification technique, along with a system to analyze the results without specialized equipment, a lateral flow assay (LFA). Using the platform developed in this study, all processes, from sample preparation to detection, can be performed without special equipment. Unlike existing PCR methods, the nucleic acid amplification can be performed in the field because hot packs do not require electricity. Thus, the designed platform can provide rapid results without the need to transport the samples to a laboratory or hospital. These advantages are not limited to operations in developing countries with poor access to medical systems. In conclusion, the developed technology is a promising tool for infectious disease management that allows for rapid identification of infectious diseases and appropriate treatment of patients.
流感病毒引起的呼吸道疾病是全球范围内严重的公共卫生问题。由于流感病毒感染的症状与严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）感染的症状相似，因此必须区分这两种病毒。因此，为了正确应对病原体，需要一种能够在医院或家中快速准确诊断的检测方法。为了满足这一需求，我们应用了环介导等温扩增（LAMP）和等温核酸扩增技术，以及一种无需专用设备即可分析结果的系统，即侧向流动测定（LFA）。使用本研究开发的平台，从样品制备到检测的所有过程都可以在没有特殊设备的情况下进行。与现有的 PCR 方法不同，核酸扩增可以在现场进行，因为不需要电的热包。因此，设计的平台可以提供快速的结果，而无需将样品运送到实验室或医院。这些优势不仅限于医疗系统不完善的发展中国家的操作。总之，开发的技术是一种有前途的传染病管理工具，可以快速识别传染病并对患者进行适当治疗。
