plays indispensable roles in maintaining self-renewal of hematopoietic stem cells.

is one of the primary demethylases responsible for reversing N6-methyladenosine (mA) modifications on mRNAs, and it plays a crucial role in many physiological and pathological processes. Previous studies have shown that is required for maintaining the function of leukemia stem cells but is dispensable for normal hematopoiesis. In this study, we found that deletion led to a moderate increase in the number of multiple progenitor cell populations while compromising the long-term self-renewal capacity of hematopoietic stem cells (HSCs). Here, we used RNA-seq and mA-seq strategies to explore the underlying molecular mechanism. At the molecular level, may regulate hematopoiesis by reducing mA modification of and maintaining gene expression levels. Overall, our study unveiled an essential role for in regulating HSC homeostasis and provides a reference for future research in this area.