Yang Bijie, Liu Yuanyuan, Xiao Feifei, Liu Zhilong, Chen Zhe, Li Zhigang, Zhou Chengfang, Kuang Mei, Shu Yi, Liu Shan, Zou Lin
Center for Clinical Molecular Medicine & Newborn Screening, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, 400014, Chongqing, PR China.
Institute of Life Sciences, Chongqing Medical University, Chongqing, China.
Open Med (Wars). 2023 Aug 9;18(1):20230766. doi: 10.1515/med-2023-0766. eCollection 2023.
is one of the primary demethylases responsible for reversing N6-methyladenosine (mA) modifications on mRNAs, and it plays a crucial role in many physiological and pathological processes. Previous studies have shown that is required for maintaining the function of leukemia stem cells but is dispensable for normal hematopoiesis. In this study, we found that deletion led to a moderate increase in the number of multiple progenitor cell populations while compromising the long-term self-renewal capacity of hematopoietic stem cells (HSCs). Here, we used RNA-seq and mA-seq strategies to explore the underlying molecular mechanism. At the molecular level, may regulate hematopoiesis by reducing mA modification of and maintaining gene expression levels. Overall, our study unveiled an essential role for in regulating HSC homeostasis and provides a reference for future research in this area.
是负责逆转mRNA上N6-甲基腺苷（mA）修饰的主要去甲基化酶之一，并且在许多生理和病理过程中发挥关键作用。先前的研究表明，对于维持白血病干细胞的功能是必需的，但对于正常造血是可有可无的。在本研究中，我们发现缺失导致多个祖细胞群体数量适度增加，同时损害造血干细胞（HSC）的长期自我更新能力。在这里，我们使用RNA测序和mA测序策略来探索潜在的分子机制。在分子水平上，可能通过减少的mA修饰并维持基因表达水平来调节造血。总体而言，我们的研究揭示了在调节HSC稳态中的重要作用，并为该领域的未来研究提供了参考。
