College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Anal Chem. 2023 Aug 29;95(34):12859-12866. doi: 10.1021/acs.analchem.3c02151. Epub 2023 Aug 17.
Cardiomyocytes are responsible for generating contractile force to pump blood throughout the body and are very sensitive to mechanical forces and can initiate mechano-electric coupling and mechano-chemo-transduction. Remarkable progress has been made in constructing heart tissue by engineered three-dimensional (3D) culture models and in recording the electrical signals of cardiomyocytes. However, it remains a severe challenge for real-time acquiring of the transient biochemical information in cardiomyocyte mechano-chemo-transduction. Herein, we reported a multifunctional platform by integrating a 3D stretchable electrochemical sensor with collagen hydrogel for the culture, electrical stimulation, and electrochemical monitoring of cardiomyocytes. The 3D stretchable electrochemical sensor was prepared by assembling functionalized conductive polymer PEDOT:PSS on an elastic scaffold, which showed excellent electrochemical sensing performance and stability under mechanical deformations. The integration of a 3D stretchable electrochemical sensor with collagen hydrogel provided an in vivo-like microenvironment for cardiomyocyte culture and promoted cell orientation via in situ electrical stimulation. Furthermore, this multifunctional platform allowed real-time monitoring of stretch-induced HO release from cardiomyocytes under their normal and pathological conditions, as well as pharmacological interventions.
心肌细胞负责产生收缩力，将血液泵送到全身，对机械力非常敏感，可以启动机械-电偶联和机械-化学转导。通过工程化的三维（3D）培养模型构建心脏组织和记录心肌细胞的电信号方面已经取得了显著的进展。然而，实时获取心肌细胞机械-化学转导中的瞬态生化信息仍然是一个严峻的挑战。在此，我们报道了一种多功能平台，该平台通过将 3D 可拉伸电化学传感器与胶原水凝胶集成，用于心肌细胞的培养、电刺激和电化学监测。3D 可拉伸电化学传感器是通过将功能化的导电聚合物 PEDOT:PSS 组装在弹性支架上制备的，该传感器在机械变形下表现出优异的电化学传感性能和稳定性。3D 可拉伸电化学传感器与胶原水凝胶的集成，为心肌细胞培养提供了类似于体内的微环境，并通过原位电刺激促进了细胞的定向生长。此外，该多功能平台允许在正常和病理条件下以及药物干预下实时监测心肌细胞拉伸诱导的 HO 释放。
