Yan Jing, Qin Yu, Fan Wen-Ting, Wu Wen-Tao, Lv Song-Wei, Yan Li-Ping, Liu Yan-Ling, Huang Wei-Hua
College of Chemistry and Molecular Sciences, Wuhan University Wuhan 430072 China
School of Pharmacy, Changzhou University Changzhou 213164 China.
Chem Sci. 2021 Oct 12;12(43):14432-14440. doi: 10.1039/d1sc04138j. eCollection 2021 Nov 10.
Recently, stretchable electrochemical sensors have stood out as a powerful tool for the detection of soft cells and tissues, since they could perfectly comply with the deformation of living organisms and synchronously monitor mechanically evoked biomolecule release. However, existing strategies for the fabrication of stretchable electrochemical sensors still face with huge challenges due to scarce electrode materials, demanding processing techniques and great complexity in further functionalization. Herein, we report a novel and facile strategy for one-step preparation of stretchable electrochemical biosensors by doping ionic liquid and catalyst into a conductive polymer (poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate), PEDOT:PSS). Bis(trifluoromethane) sulfonimide lithium salt as a small-molecule plasticizer can significantly improve the stretchability and conductivity of the PEDOT:PSS film, and cobalt phthalocyanine as an electrocatalyst endows the film with excellent electrochemical sensing performance. Moreover, the functionalized PEDOT:PSS retained good cell biocompatibility with two extra dopants. These satisfactory properties allowed the real-time monitoring of stretch-induced transient hydrogen peroxide release from cells. This work presents a versatile strategy to fabricate conductive polymer-based stretchable electrodes with easy processing and excellent performance, which benefits the in-depth exploration of sophisticated life activities by electrochemical sensing.
近年来，可拉伸电化学传感器作为检测软细胞和组织的有力工具脱颖而出，因为它们能够完美地顺应生物体的变形，并同步监测机械诱发的生物分子释放。然而，由于电极材料稀缺、加工技术要求高以及进一步功能化的复杂性，现有的可拉伸电化学传感器制造策略仍面临巨大挑战。在此，我们报道了一种通过将离子液体和催化剂掺杂到导电聚合物（聚（3,4 - 乙撑二氧噻吩）：聚（苯乙烯磺酸盐），PEDOT:PSS）中一步制备可拉伸电化学生物传感器的新颖简便策略。双（三氟甲烷）磺酰亚胺锂盐作为小分子增塑剂可显著提高PEDOT:PSS膜的拉伸性和导电性，而钴酞菁作为电催化剂赋予该膜优异的电化学传感性能。此外，功能化的PEDOT:PSS与两种额外的掺杂剂保持了良好的细胞生物相容性。这些令人满意的特性使得能够实时监测细胞拉伸诱导的瞬时过氧化氢释放。这项工作提出了一种通用策略，用于制造具有易于加工和优异性能的基于导电聚合物的可拉伸电极，这有利于通过电化学传感深入探索复杂的生命活动。
