Silbert A M, Baum J L
Arch Ophthalmol. 1979 Jun;97(6):1141-3. doi: 10.1001/archopht.1979.01020010595019.
Electron microscopic studies have offered presumptive evidence that the origin of both cellular and extracellular components of the retrocorneal membrane (RCM) derive from corneal endothelial cells. In an attempt to demonstrate conclusively the origin of the cell in the RCM, we performed 8-mm exchange, penetrating keratoplasties between male and female rabbits. After allowing for complete healing and using only clear corneas, we produced central RCM by freezing the central cornea after inducing intraocular inflammation. Care was taken to ensure that the membrane was surrounded by normal donor endothelium and Descemet's membrane was intact. Sex chromatin counts of the cells in the RCM of a female host with a male graft showed an average of 2% Barr bodies. Cells in the membrane of a male host with a female graft showed an average of 40% Barr bodies. The experimental and control sex chromatin counts were almost identical. This study provides conclusive evidence that central retrocorneal, fibroblast-like cells and membranes in rabbits are derived from corneal endothelial cells.
电子显微镜研究提供了推测性证据，表明角膜后弹力层（RCM）的细胞和细胞外成分均起源于角膜内皮细胞。为了最终证实RCM中细胞的起源，我们在雄性和雌性兔子之间进行了8毫米的穿透性角膜移植交换手术。在完全愈合且仅使用透明角膜后，我们通过在诱导眼内炎症后冷冻中央角膜来产生中央RCM。注意确保该膜被正常的供体内皮细胞包围，并且Descemet膜完整无损。在接受雄性移植物的雌性宿主的RCM中，细胞的性染色质计数显示平均有2%的巴氏小体。在接受雌性移植物的雄性宿主的膜中，细胞显示平均有40%的巴氏小体。实验和对照的性染色质计数几乎相同。这项研究提供了确凿的证据，表明兔子的中央角膜后弹力层、成纤维细胞样细胞和膜起源于角膜内皮细胞。
