Wolfe J A, Stuck B E, Schuschereba S T, Fox L P
Doc Ophthalmol. 1985 Apr 30;59(3):277-99. doi: 10.1007/BF00159265.
A moderately severe thermal injury of the central cornea of 48 Dutch-belted rabbit eyes was produced with a carbon (CO2) laser. The lesions were photographed with a slit lamp (SL) camera immediately following the injury and at 1, 2, 4, 7, 14, 21, 30 and 60 days after the exposure. Lesion size, opaqueness, and depth were graded clinically by SL biomicroscopy at the same intervals. No significant differences were found (p less than or equal to 0.05) between groups of eyes treated with flurbiprofen (0.03%), prednisolone acetate (1%), and vehicle control four-times-a-day for three weeks following injury. Additionally, eyes were studied histopathologically at 3 and 60 days following injury by light and transmission electron microscopy, and clinically at 30 and 60 days by endothelial specular microscopy. Important clinical and histopathological findings included coagulative necrosis of the corneal epithelium, epithelial sloughing, fusion of stromal collagen, stromal edema and inflammatory cell infiltration, stromal scar formation, corneal thinning, endothelial hyperplasia and metaplasia, fibrinous anterior chamber reaction with hypopyon, and retrocorneal fibrous membrane formation.
使用二氧化碳（CO2）激光对48只荷兰带兔眼的中央角膜造成中度严重的热损伤。损伤后立即以及暴露后1、2、4、7、14、21、30和60天，用裂隙灯（SL）相机对损伤部位进行拍照。在相同时间间隔通过SL生物显微镜对损伤大小、混浊程度和深度进行临床分级。损伤后连续三周每天四次用氟比洛芬（0.03%）、醋酸泼尼松龙（1%）和赋形剂对照治疗的各组兔眼之间未发现显著差异（p≤0.05）。此外，在损伤后3天和60天通过光镜和透射电子显微镜进行组织病理学研究，在30天和60天通过内皮细胞镜面显微镜进行临床研究。重要的临床和组织病理学发现包括角膜上皮凝固性坏死、上皮脱落、基质胶原融合、基质水肿和炎性细胞浸润、基质瘢痕形成、角膜变薄、内皮细胞增生和化生、伴有前房积脓的纤维素性前房反应以及角膜后纤维膜形成。
