Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, Veracruz, Mexico.
División de Estudios de Posgrado e Investigación, Tecnológico Nacional de México/Instituto Tecnológico de Orizaba, Orizaba, Veracruz, Mexico.
PLoS One. 2023 Aug 17;18(8):e0289972. doi: 10.1371/journal.pone.0289972. eCollection 2023.
Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involved in AD which generates a low methane yield. This study aimed to improve the methane yield of pelagic Sargassum, using different energy-saving pretreatments and identifying the microbial community associated with methane production. We applied different energy-saving pretreatments to algal biomass and assessed the methane yield using a biomethane potential (BMP) test. The microbial communities involved in the AD of the best- and worst-performing methanogenic systems were analyzed by high-throughput sequencing. The results showed that pretreatment modified the content of inorganic compounds, fibers, and the C:N ratio, which had a strong positive correlation with BMP. The water washing pretreatment resulted in the best methane yield, with an increase of 38%. DNA metabarcoding analysis revealed that the bacterial genera Marinilabiliaceae_uncultured, DMER64, Treponema, and Hydrogenispora, as well as the archaea genera Methanosarcina, RumEn_M2, Bathyarchaeia, and Methanomassiliicocus, dominated the microbial community with a high methane yield. This study is the first to demonstrate the microbial community structure involved in the AD of Sargassum spp. The pretreatments presented in this study can help overcome the limitations associated with methane yield.
马尾藻属大量繁殖冲击加勒比海沿岸，破坏当地经济和环境。厌氧消化（AD）已被提议作为将大型藻类转化为有价值资源的一种有吸引力的选择。马尾藻属的组成复杂，影响 AD 过程中涉及的微生物组成，导致甲烷产量低。本研究旨在通过不同的节能预处理方法提高中上层马尾藻的甲烷产量，并确定与甲烷生成相关的微生物群落。我们对藻类生物质应用了不同的节能预处理方法，并通过生物甲烷潜力（BMP）测试评估甲烷产量。通过高通量测序分析了 AD 中表现最好和最差的产甲烷系统的微生物群落。结果表明，预处理改变了无机化合物、纤维和 C：N 比的含量，与 BMP 呈强正相关。水洗预处理的甲烷产量最高，增加了 38%。DNA 宏条形码分析显示，具有高甲烷产量的微生物群落主要由 Marinilabiliaceae_uncultured、DMER64、Treponema 和 Hydrogenispora 等细菌属，以及 Methanosarcina、RumEn_M2、Bathyarchaeia 和 Methanomassiliicocus 等古菌属组成。本研究首次证明了 AD 中马尾藻属的微生物群落结构。本研究中提出的预处理方法可以帮助克服与甲烷产量相关的限制。
