Faculty of Science and Engineering, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.
Faculty of Science and Engineering, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.
Bioresour Technol. 2021 Jun;330:124962. doi: 10.1016/j.biortech.2021.124962. Epub 2021 Mar 11.
This study aimed to investigate the effect of bioaugmentation on microbial community and function in a continuous anaerobic process treating lignocellulosic cow manure. One reactor (R) received bioaugmentation dosage for a certain period (d100-d170) and stopped afterward (d170-d220), while the same applied to the control (R) except sterilized bioaugmentation dosage was introduced. Samples were taken on day130, 170 and 220 from both reactors for metatranscriptomic analysis. The results underlined the promotive effect of bioaugmentation on indigenous microorganisms regarding hydrolysis and methanogenesis. Bioaugmentation contributed to the enrichment of Clostridium, Cellvibrio, Cellulomonas, Bacillus, Fibrobacter, resulting in enhanced cellulase activity (R: 0.917-1.081; R: 0.551-0.677). Moreover, bioaugmentation brought R the prosperity of uncultured_ Bathyarchaeia, a prominent archaeal group responsible for the improved methyl-coenzyme M reductase activity, thus accelerated methanogenesis. Unique metabolic pathways (autotrophic carbon fixation and methanogenesis) in uncultured_ Bathyarchaeia broadened the horizon of its fundamental role as acetogens and methanogens in anaerobic digestion.
本研究旨在探讨生物强化对连续厌氧处理木质纤维素牛粪过程中微生物群落和功能的影响。一个反应器（R）在一定时期（d100-d170）内接受生物强化剂量，之后停止（d170-d220），而对照（R）则相同，只是引入了灭菌的生物强化剂量。在第 130、170 和 220 天从两个反应器中取样进行宏转录组分析。结果强调了生物强化对水解和产甲烷过程中土著微生物的促进作用。生物强化有助于丰富梭菌、纤维弧菌、纤维单胞菌、芽孢杆菌、纤维杆菌，从而提高纤维素酶活性（R：0.917-1.081；R：0.551-0.677）。此外，生物强化使 R 中未培养的 Bathyarchaeia 大量繁殖，这是一个负责提高甲基辅酶 M 还原酶活性的重要古菌群，从而加速了产甲烷作用。未培养的 Bathyarchaeia 中的独特代谢途径（自养碳固定和产甲烷作用）拓宽了其作为厌氧消化中乙酸生成菌和产甲烷菌的基本作用的视野。
