Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Science. 2023 Aug 18;381(6659):748-753. doi: 10.1126/science.adf3345. Epub 2023 Aug 17.
During the consumption of alkanes, will form a biofilm around an oil droplet, but the role this plays during degradation remains unclear. We identified a shift in biofilm morphology that depends on adaptation to oil consumption: Longer exposure leads to the appearance of dendritic biofilms optimized for oil consumption effected through tubulation of the interface. In situ microfluidic tracking enabled us to correlate tubulation to localized defects in the interfacial cell ordering. We demonstrate control over droplet deformation by using confinement to position defects, inducing dimpling in the droplets. We developed a model that elucidates biofilm morphology, linking tubulation to decreased interfacial tension and increased cell hydrophobicity.
在烷烃的消耗过程中， 会在油滴周围形成生物膜，但它在降解过程中所起的作用尚不清楚。我们发现生物膜形态的变化取决于对油消耗的适应：更长时间的暴露导致出现了枝状生物膜，这种生物膜通过管化界面来优化油消耗。原位微流控跟踪使我们能够将管化与界面细胞排列的局部缺陷相关联。我们通过使用限制来定位缺陷来证明对液滴变形的控制，从而在液滴中产生凹陷。我们开发了一个模型，阐明了生物膜形态，将管化与界面张力降低和细胞疏水性增加联系起来。
