Department of Orthopedics Surgery, Tongde Hospital of Zhejiang Province.
Department of Orthopedics Surgery, Tongde Hospital of Zhejiang Province;
J Vis Exp. 2023 Jul 21(197). doi: 10.3791/64797.
Decellularized cartilage extracellular matrix (DC-ECM) hydrogels are promising biomaterials for tissue engineering and regenerative medicine due to their biocompatibility and ability to mimic natural tissue properties. This protocol aims to produce DC-ECM hydrogels that closely mimic the native ECM of cartilage tissue. The protocol involves a combination of physical and chemical disruption and enzymatic digestion to remove the cellular material while preserving the structure and composition of the ECM. The DC-ECM is cross-linked using a chemical agent to form a stable and biologically active hydrogel. The DC-ECM hydrogel has excellent biological activity, spatial structure, and biological induction function, as well as low immunogenicity. These characteristics are beneficial in promoting cell adhesion, proliferation, differentiation, and migration and for creating a superior microenvironment for cell growth. This protocol provides a valuable resource for researchers and clinicians in the field of tissue engineering. Biomimetic hydrogels can potentially enhance the development of effective tissue engineering strategies for cartilage repair and regeneration.
去细胞软骨细胞外基质（DC-ECM）水凝胶由于其生物相容性和模拟天然组织特性的能力，是组织工程和再生医学有前途的生物材料。本方案旨在制备尽可能模拟软骨组织天然 ECM 的 DC-ECM 水凝胶。该方案涉及物理和化学破坏以及酶消化的组合，以去除细胞材料，同时保留 ECM 的结构和组成。使用化学试剂交联 DC-ECM 以形成稳定且具有生物活性的水凝胶。DC-ECM 水凝胶具有优异的生物活性、空间结构和生物诱导功能，以及较低的免疫原性。这些特性有利于促进细胞黏附、增殖、分化和迁移，并为细胞生长创造优越的微环境。该方案为组织工程领域的研究人员和临床医生提供了有价值的资源。仿生水凝胶有可能增强用于软骨修复和再生的有效组织工程策略的发展。
