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利用新一代测序方法开发DNA标记用于对光果甘草（Glycyrrhiza glabra L.）和（Willd.）Miers进行分子鉴定

Development of DNA markers using next-generation sequencing approach for molecular authentication of L. and (Willd.) Miers.

作者信息

Sharma Anu Radha, Vohra Manik, Vinay Chigateri M, Paul Bobby, Chakrabarty Sanjiban, Rai Padmalatha S

机构信息

Department of Biotechnology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104 India.

Department of Bioinformatics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.

出版信息

3 Biotech. 2023 Sep;13(9):304. doi: 10.1007/s13205-023-03732-7. Epub 2023 Aug 15.

DOI:10.1007/s13205-023-03732-7
PMID:37593204
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10427588/
Abstract

UNLABELLED

The adulteration of plants and their materials used in herbal formulations poses a severe health concern. Hence, there is a need to establish a reliable, cost-effective, and robust molecular biomarker to distinguish among species and identify herbal plants and raw drugs from adulterants. The present study used suppressive subtractive hybridization and next-generation sequencing technology to identify novel DNA markers for  L. and  (Willd.) Miers We identified two primer sets for and one for  The DNA markers were validated in different accessions of  and  common adulterants to determine the sensitivity and specificity of developed DNA markers. The designed DNA markers showed 100% sensitivity and specificity in detecting  and  from their adulterants. The strategy described here can be extrapolated for developing DNA markers to authenticate other plant species.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-023-03732-7.

摘要

未标记

草药配方中使用的植物及其原料的掺假对健康构成严重威胁。因此，需要建立一种可靠、经济高效且强大的分子生物标志物，以区分不同物种，并从掺假物中识别草药植物和原料药。本研究使用抑制性消减杂交和下一代测序技术，为[具体物种1]和[具体物种2]鉴定新型DNA标记。我们为[具体物种1]鉴定了两组引物，为[具体物种2]鉴定了一组引物。在[具体物种1]和[具体物种2]的不同种质以及常见掺假物中验证了这些DNA标记，以确定所开发DNA标记的敏感性和特异性。所设计的DNA标记在从掺假物中检测[具体物种1]和[具体物种2]时显示出100%的敏感性和特异性。这里描述的策略可用于开发DNA标记以鉴定其他植物物种。

补充信息

在线版本包含可在10.1007/s13205-023-03732-7获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/b2f5cef4255b/13205_2023_3732_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/f78fd2cbff0a/13205_2023_3732_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/72e0f8837cb5/13205_2023_3732_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/0842c55eceb0/13205_2023_3732_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/9764bad3bc79/13205_2023_3732_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/0758e6aae10c/13205_2023_3732_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/b2f5cef4255b/13205_2023_3732_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/f78fd2cbff0a/13205_2023_3732_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/72e0f8837cb5/13205_2023_3732_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/0842c55eceb0/13205_2023_3732_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/9764bad3bc79/13205_2023_3732_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/0758e6aae10c/13205_2023_3732_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/868f/10427588/b2f5cef4255b/13205_2023_3732_Fig6_HTML.jpg

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2
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Pharmacogn Mag. 2016 May;12(Suppl 3):S384-8. doi: 10.4103/0973-1296.185780.
3
Suppression Subtractive Hybridization Versus Next-Generation Sequencing in Plant Genetic Engineering: Challenges and Perspectives.
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Mol Biotechnol. 2015 Oct;57(10):880-903. doi: 10.1007/s12033-015-9884-z.
4
Phytochemical, therapeutic, and ethnopharmacological overview for a traditionally important herb: Boerhavia diffusa Linn.一种传统上重要的草药——白花丹的植物化学、治疗作用及民族药理学概述
Biomed Res Int. 2014;2014:808302. doi: 10.1155/2014/808302. Epub 2014 May 14.
5
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PLoS One. 2013 Oct 3;8(10):e76925. doi: 10.1371/journal.pone.0076925. eCollection 2013.
6
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9
Sequencing technologies and genome sequencing.测序技术和基因组测序。
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