Laboratorio de Fisiología de Crustáceos, Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Instituto de Biodiversidad Y Biología Experimental Y Aplicada (IBBEA), Universidad de Buenos Aires. CONICET, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina.
Laboratorio de Biología de La Reproducción, Crecimiento Y Nutrición de Crustáceos Decápodos, Instituto de Biodiversidad Y Biología Experimental Y Aplicada (IBBEA). Facultad de Ciencias Exactas Y Naturales, Departamento de Biodiversidad y Biología Experimental,, Universidad de Buenos Aires, CONICET, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina.
Arch Environ Contam Toxicol. 2023 Aug;85(2):181-190. doi: 10.1007/s00244-023-01027-9. Epub 2023 Aug 18.
The current study was aimed at studying the long-term effects of diclofenac on the freshwater shrimp Neocaridina davidi, concerning survival, somatic growth, and reproduction. In this study, both ovigerous females and males of this species were exposed for 63 d to 0 (control), 0.1, or 1 mg/L of diclofenac. At the highest concentration, significant mortality was detected, and the somatic growth of females was significantly decreased. The percentage of females with a second spawn, observable from day 45, significantly increased at 1 mg/L, while the time between spawns was significantly reduced at both concentrations assayed. However, the gonadal analysis made at the end of the assay in the surviving females showed a significantly lower proportion of advanced oocytes in females exposed to 1 mg/L, as compared to control. Concerning hatching, the percentage of ovigerous females that could have successful hatching was reduced at 1 mg/L of diclofenac, especially for the first spawn. For the second spawn, the low number of juveniles hatched from females exposed to 1 mg/L also showed a significantly higher incidence of morphological abnormalities, such as hydropsy and underdeveloped appendages. Taken together, these results showed that even when diclofenac was able to produce earlier spawns, the reproductive output of each spawn was reduced.
本研究旨在研究双氯芬酸对淡水虾 Neocaridina davidi 的长期影响，包括生存、体生长和繁殖。在这项研究中，该物种的抱卵雌虾和雄虾分别暴露于 0（对照）、0.1 或 1mg/L 的双氯芬酸中 63 天。在最高浓度下，检测到显著的死亡率，并且雌性的体生长显著降低。从第 45 天开始，可观察到 1mg/L 时具有第二产卵的雌性的比例显著增加，而两次产卵之间的时间在两种测定浓度下均显著缩短。然而，在幸存雌性中进行的终末性腺分析显示，与对照组相比，暴露于 1mg/L 的雌性中具有高级卵母细胞的比例显著降低。关于孵化，在 1mg/L 双氯芬酸作用下，能够成功孵化的抱卵雌性的比例降低，尤其是第一产卵。对于第二产卵，暴露于 1mg/L 的雌虾孵化出的幼虾数量较少，且畸形发生率显著升高，如水肿和附肢发育不良。综上所述，这些结果表明，即使双氯芬酸能够产生更早的产卵，每个产卵的繁殖输出也会减少。
