Escribano Begoña M, Muñoz-Jurado Ana, Luque Evelio, Galván Alberto, LaTorre Manuel, Caballero-Villarraso Javier, Giraldo Ana I, Agüera Eduardo, Túnez Isaac
Department of Cell Biology, Physiology and Immunology, Faculty of Veterinary Medicine, University of Cordoba, Spain; Maimonides Institute for Research in Biomedicine of Cordoba (IMIBIC), Cordoba, Spain.
Department of Cell Biology, Physiology and Immunology, Faculty of Veterinary Medicine, University of Cordoba, Spain.
Neuroscience. 2023 Oct 1;529:116-128. doi: 10.1016/j.neuroscience.2023.08.005. Epub 2023 Aug 16.
Oxidative stress is heavily involved in several pathological features of Multiple Sclerosis (MS), such as myelin destruction, axonal degeneration, and inflammation. Different therapies have been shown to reduce the oxidative stress that occurs in the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Some of these therapies are transcranial magnetic stimulation (TMS), extra virgin olive oil (EVOO) and S-allyl cysteine (SAC). This study aims to test the antioxidant effect of these three therapies, to compare the efficacy of SAC versus TMS and EVOO, and to analyze the effect of combining SAC + TMS and SAC and EVOO. Seventy Dark Agouti rats were used, which were divided into Control group; Vehicle group; Mock group; SAC; EVOO; TMS; SAC + EVOO; SAC + TMS; EAE; EAE + SAC; EAE + EVOO; EAE + TMS; EAE + SAC + EVOO; EAE + SAC + TMS. The TMS consisted of an oscillatory magnetic field in the form of a sine wave with a frequency of 60 Hz and an amplitude of 0.7mT (EL-EMF) applied for two hours in the morning, once a day, five days a week. SAC was administered at a dose of 50 mg/kg body weight, orally daily, five days a week. EVOO represented 10% of their calorie intake in the total standard daily diet of rats AIN-93G. All treatments were maintained for 51 days. TMS, EVOO and SAC, alone or in combination, reduce oxidative stress, increasing antioxidant defenses and also lowering the clinical score. Combination therapies do not appear to be more potent than individual therapies against the oxidative stress of EAE or its clinical symptoms.
氧化应激与多发性硬化症（MS）的多种病理特征密切相关，如髓鞘破坏、轴突退化和炎症。已证明不同疗法可减轻MS动物模型实验性自身免疫性脑脊髓炎（EAE）中出现的氧化应激。其中一些疗法包括经颅磁刺激（TMS）、特级初榨橄榄油（EVOO）和S-烯丙基半胱氨酸（SAC）。本研究旨在测试这三种疗法的抗氧化作用，比较SAC与TMS和EVOO的疗效，并分析联合使用SAC + TMS以及SAC和EVOO的效果。使用了70只深色刺豚鼠，将其分为对照组；赋形剂组；假手术组；SAC组；EVOO组；TMS组；SAC + EVOO组；SAC + TMS组；EAE组；EAE + SAC组；EAE + EVOO组；EAE + TMS组；EAE + SAC + EVOO组；EAE + SAC + TMS组。TMS由频率为60Hz、振幅为0.7mT 的正弦波形式的振荡磁场（EL-EMF）组成，每天上午施加两小时，每周五天。SAC以50mg/kg体重的剂量口服给药，每天一次，每周五天。EVOO在大鼠AIN-93G的每日标准总饮食中占其卡路里摄入量的10%。所有治疗持续51天。TMS、EVOO和SAC单独或联合使用均可降低氧化应激，增强抗氧化防御能力并降低临床评分。联合疗法在对抗EAE的氧化应激或其临床症状方面似乎并不比单一疗法更有效。
