Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Gronostajowa 7, 30-387, Kraków, Poland.
Malopolska Centre of Biotechnology in Krakow, 30-387, Kraków, Poland.
Sci Rep. 2023 Aug 18;13(1):13434. doi: 10.1038/s41598-023-40729-x.
Skeletal muscle regeneration relies on the reciprocal interaction between many types of cells. Regenerative capacity may be altered in different disorders. In our study, we investigated whether the deletion of miR-378a (miR-378) affects muscle regeneration. We subjected 6-week-old wild-type (WT) and miR-378 knockout (miR-378) animals to the glycerol-induced muscle injury and performed analyses in various time-points. In miR-378 animals, an elevated abundance of muscle satellite cells (mSCs) on day 3 was found. Furthermore, fibro-adipogenic progenitors (FAPs) isolated from the muscle of miR-378 mice exhibited enhanced adipogenic potential. At the same time, lack of miR-378 did not affect inflammation, fibrosis, adipose tissue deposition, centrally nucleated fiber count, muscle fiber size, FAP abundance, and muscle contractility at any time point analyzed. To conclude, our study revealed that miR-378 deletion influences the abundance of mSCs and the adipogenic potential of FAPs, but does not affect overall regeneration upon acute, glycerol-induced muscle injury.
骨骼肌再生依赖于多种细胞的相互作用。在不同的疾病中，再生能力可能会发生改变。在我们的研究中，我们调查了 miR-378a（miR-378）的缺失是否会影响肌肉再生。我们对 6 周龄的野生型（WT）和 miR-378 敲除（miR-378）动物进行了甘油诱导的肌肉损伤，并在不同时间点进行了分析。在 miR-378 动物中，第 3 天发现肌肉卫星细胞（mSCs）的丰度升高。此外，从 miR-378 小鼠肌肉中分离出的成纤维脂肪祖细胞（FAP）表现出增强的成脂潜能。与此同时，在任何分析的时间点，miR-378 的缺失都不会影响炎症、纤维化、脂肪组织沉积、中央核纤维计数、肌纤维大小、FAP 丰度和肌肉收缩力。总之，我们的研究表明，miR-378 的缺失影响 mSCs 的丰度和 FAP 的成脂潜能，但不会影响甘油诱导的急性肌肉损伤后的整体再生。
