Hausman G J, Kauffman R G
J Anim Sci. 1986 Aug;63(2):659-73. doi: 10.2527/jas1986.632659x.
Tritiated thymidine autoradiography and histochemistry were used to study the development of subcutaneous adipose tissue of lean and obese fetuses and postnatal pigs. A pattern of tritiated thymidine uptake by pre-adipocytes and adipocyte lipid accumulation was demonstrated during the growth of the fetal pig. In the youngest fetuses there was a period of intense stromal cell mitotic activity before any adipocyte lipid accumulation. During subsequent fetal development, clusters of tightly arranged stromal cells were formed. Lipid accumulation occurred only in these cell clusters. During this time of cell cluster formation and lipid accumulation, mitotic activity was minimal. In obese fetuses, stromal cell mitotic activity overlapped temporarily with the cell cluster formation and lipid accumulation period. In early postnatal pigs, fat cell clusters increased in size until they "physically filled" the adipose tissue. In pigs 3 d and older, there was extensive mitotic activity of cells within the fat cell clusters. The synthesis of this second bed of pre-adipocytes and the altered developmental pattern in the obese fetuses is suggested to be due to the influence of a high fat diet. The significance of these findings in terms of plausible links between pre-adipocyte mitosis and lipid accumulation is discussed.
利用氚标记胸腺嘧啶核苷放射自显影术和组织化学方法，研究了瘦型和肥胖型胎儿及出生后仔猪皮下脂肪组织的发育情况。在胎猪生长过程中，观察到前脂肪细胞摄取氚标记胸腺嘧啶核苷以及脂肪细胞脂质积累的模式。在最幼小的胎儿中，在任何脂肪细胞脂质积累之前，存在一段强烈的基质细胞有丝分裂活动期。在随后的胎儿发育过程中，形成了紧密排列的基质细胞簇。脂质积累仅发生在这些细胞簇中。在细胞簇形成和脂质积累期间，有丝分裂活动极少。在肥胖胎儿中，基质细胞有丝分裂活动与细胞簇形成和脂质积累期暂时重叠。在出生后早期的仔猪中，脂肪细胞簇不断增大，直至它们“实际填满”脂肪组织。在3日龄及以上的仔猪中，脂肪细胞簇内的细胞有广泛的有丝分裂活动。肥胖胎儿中前脂肪细胞的第二次增殖以及发育模式的改变，被认为是由于高脂饮食的影响。本文讨论了这些发现对于前脂肪细胞有丝分裂与脂质积累之间可能联系的意义。
