Verdery R B, Nist C, Fujimoto W Y, Wight T N, Glomset J A
In Vitro. 1981 Nov;17(11):956-64. doi: 10.1007/BF02618420.
Prolonged maintenance of human dermal fibroblasts in MCDB-104 medium supplemented with pooled human serum or platelet factor deficient preparations of human serum led to appearance of a large number of membrane bound inclusions, resembling lysosomes, and proliferation of small, Golgi associated vesicles. These inclusions did not appear if the cells were grown in Dulbecco-Vogt's modification of Eagle's minimal essential medium or in minimal essential medium supplemented with the same human serum fractions. Cells that acquired inclusions during a 10 d incubation in MCDB-104 subsequently lost these inclusions when transferred to Dulbecco'Vogt's medium for 4 d. Similar reversal of effects of MCDB-104 was also produced by MCDB-104 buffered with bicarbonate instead of HEPES.
在补充有混合人血清或人血清血小板因子缺陷制剂的MCDB - 104培养基中长时间培养人皮肤成纤维细胞，会导致出现大量类似溶酶体的膜结合内含物，以及小的、与高尔基体相关的囊泡增殖。如果细胞在杜尔贝科 - 沃格特改良的伊格尔最低必需培养基中生长，或在补充相同人血清成分的最低必需培养基中生长，则不会出现这些内含物。在MCDB - 104中孵育10天期间获得内含物的细胞，随后转移到杜尔贝科 - 沃格特培养基中培养4天时，会失去这些内含物。用碳酸氢盐而非HEPES缓冲的MCDB - 104也会产生类似的MCDB - 104效应逆转。
