Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, People's Republic of China.
Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, People's Republic of China.
J Proteomics. 2023 Sep 30;288:104994. doi: 10.1016/j.jprot.2023.104994. Epub 2023 Aug 19.
Low temperature is a crucial environmental factor limiting the productivity and distribution of banana. Ubiquitination (Kub) is one of the main posttranslational modifications (PTMs) involved in plant responses to abiotic stresses. However, little information is available on the effects of Kub on banana under cold stress. In this study, we used label-free quantification (LFQ) to identify changes in the protein expression and Kub levels in banana seedling leaves after chilling treatment. In total, 4156 proteins, 1089 ubiquitinated proteins and 2636 Kub sites were quantified. Western blot assays showed that Kub was abundant in leaves after low-temperature treatment. Our results show that the proteome and ubiquitylome were negatively correlated, indicating that Kub could be involved in the degradation of proteins in banana after chilling treatment. Based on bioinformatics analysis, low-temperature stress-related signals and metabolic pathways such as cold acclimation, glutathione metabolism, calcium signaling, and photosynthesis signaling were identified. In addition, we found that transcription factors and chromatin remodeling factors related to low-temperature stress were ubiquitinated. Overall, our work presents the first systematic analysis of the Kub proteome in banana under cold stress and provides support for future studies on the regulatory mechanisms of Kub during the cold stress response in plants. SIGNIFICANCE: Banana is a typical tropical fruit tree with poor low-temperature tolerance,however, the role of PTMs such as Kub in the cold response of banana remains unclear. This study highlights the fact that the effects of low-temperature on proteome and ubiquitylome in the banana seedling leaves, we discussed the correlation between transcriptome and proteome, ubiquitylome and proteome, and we analyzed the expression and the changes of ubiquitination levels of low-temperature related proteins and pathway after chilling treatment, and we found that transcription factors and chromatin remodeling factors related to low-temperature stress were ubiquitinated. This study provides new insights into the ubiquitination pathway of banana under cold stress.
低温是限制香蕉生产力和分布的关键环境因素。泛素化（Kub）是植物响应非生物胁迫的主要翻译后修饰（PTMs）之一。然而，关于 Kub 在香蕉冷胁迫下的影响，信息很少。在这项研究中，我们使用无标记定量（LFQ）来鉴定冷处理后香蕉幼苗叶片中蛋白质表达和 Kub 水平的变化。总共定量了 4156 种蛋白质、1089 种泛素化蛋白质和 2636 个 Kub 位点。Western blot 检测显示，低温处理后叶片中 Kub 含量丰富。结果表明，低温胁迫下的蛋白质组和泛素组呈负相关，表明 Kub 可能参与了冷胁迫后香蕉中蛋白质的降解。基于生物信息学分析，鉴定到了低温应激相关信号和代谢途径，如冷驯化、谷胱甘肽代谢、钙信号和光合作用信号。此外，我们发现与低温应激相关的转录因子和染色质重塑因子被泛素化。总之，本研究首次系统分析了冷胁迫下香蕉中的 Kub 蛋白质组，为进一步研究 Kub 在植物冷应激响应中的调控机制提供了支持。
